TPA处理增强喉鳞状细胞癌细胞株Hep-2细胞的增殖  

TPA increases cell proliferation of HEp-2 cell line in vitro

作　　者：刘巍巍[1] 曾宗渊[2] 肖锡宾[3] 

机构地区：[1]中山大学肿瘤防治中心综合三科,广州510060 [2]中山大学肿瘤防治中心头颈科 [3]中山大学肿瘤防治中心中心实验室

出　　处：《临床耳鼻咽喉科杂志》2005年第17期788-789,共2页Journal of Clinical Otorhinolaryngology

摘　　要：目的:探讨TPA对体外培养的人喉鳞状细胞癌细胞株HEp-2细胞的作用.方法:应用不同浓度TPA处理体外培养的HEp-2细胞,MTT法检测HEp-2细胞的存活比率.结果:HEp-2细胞经TPA处理后其细胞增殖能力呈现峰型,高峰出现在10 μg/L水平上,此时处理细胞的增殖能力是对照组的1.3～1.4倍.随着TPA浓度的增高,HEp-2细胞的增殖能力逐渐下降.结论:TPA处理可以增强HEp-2细胞的增殖能力.Objective：To investigate the influence of TPA treatment on HEp-2 cell line in vitro. Method： HEp-2 cell line cultured in vitro was treated by different concentration levels of TPA. MTT method was used to detect survival cell ratio of HEp-2. Result：Cell growth of HEp-2 after treatment of TPA manifested an peak. The highest peak which was 1.3 to 1.4 times of control cell presented on 10μg/L concentration level. Cell proliferation ability dropped gradually following increase of TPA concentration. Conclusions： TPA can increase cell proliferation of HEp-2 cell in vitro.

关 键 词：TPA 喉肿瘤 HEP-2细胞株 细胞增殖 

分 类 号：R739.65[医药卫生—肿瘤]