红螯螯虾雄性生殖系统的生化组成及精子代谢  

Biochemical composition and sperm metabolism in male reproductive system of crayfish,Cherax quadricarinatus

作　　者：丁银娣[1] 王群[1] 李恺[1] 陈东华[1] 

机构地区：[1]华东师范大学生命科学学院,上海200062

出　　处：《中国水产科学》2005年第5期562-568,共7页Journal of Fishery Sciences of China

基　　金：国家自然科学基金资助项目(30300265)

摘　　要：通过对红螯螯虾(Cherax quadricarinatus)雄性生殖系统各组织的生化测定和乳酸脱氢酶(LDH)、琥珀酸脱氢酶(SDH)、延胡索酸还原酶(FR)的活性测定发现,红螯螯虾精荚(含精子)中的糖类和蛋白质含量丰富,分别为18.264和12.846 mg/g(FW),总脂含量相对较低,仅为1.613 mg/g(FW);精子(含初级精荚壁)亦以糖类和蛋白质含量较高,分别达到5.227和8.475 mg/g(FW),总脂含量因过低而未能测出,但精子的上述物质含量均显著低于精荚。酶活性测定发现,LDH酶活性以精巢、输精管和射精管较高,其中又以前输精管最高,为1 426.258 U/(g protein),可能与其功能相关;精子和精荚LDH活性极显著低于输精管,含量分别为87.375和36.215 U/(g protein),两者间差异显著;FR活性在精荚和精子中较高,两者分别达到19.945和15.732 U/(mg protein),显著高于生殖系统其他组织器官,但均未检测到SDH酶活性,故其SDH/FR的比值趋向于0。而生化物质的组织化学以及酶组化定位的结果进一步证实了上述结果。此外,LDH同工酶酶谱结果证实,精荚仅含有一条厌氧型LDHA编码亚基占优势的同工酶谱带。所有实验结果表明,红螯螯虾精子代谢的方式以厌氧型为主,其代谢底物主要为糖类,为厌氧型糖酵解,其代谢底物由次级精荚壁提供。The sperms of the Crustacea are often atypical, bizarre, and non-flagellated, so it is difficult to investigate the sperm metabolism directly. Biochemical studies, as well as enzyme assays for lactate dehydrogenase （LDH） activity, succinate dehydrogenase （SDH） activity and fumarate reductase （FR） activity, have been conducted on the male reproductive tissue in order to provide reference to sperm metabolism in the male crayfish, Cherax quadricarinatus. The results reveal that spermatophores （with sperm inside） contain high levels of carbohydrate and protein, which are （18.264 ± 0.081） and （12.846 ± 2.179） mg/g of wet tissue weight, respectively, but low level of total lipid, （1.613 ± 0.026） mg/g of wet tissue weight. Compared with other reproductive tissues, the corresponding contents in sperm （with the primer layer of spermatophore） are fairly low, the content of total lipid in which is too low to be detected; in contrast, there are still high levels of carbohydrate and protein, （5.227 ± 0.054） and （8.475 ± 0.943） mg/g of wet tissue weight, respectively, all significantly lower than those in spermatophore. Enzyme assays show that the lactate dehydrogenase activity is high in testis, vas deferens and ejaculatory duct, particularly in the proximal vas deferens （1 426.258 ± 329.260） U/g protein, in agreement with its function. Secondly, the lactate dehydrogenase activities in sperm and spermatophore are significantly lower than those in the vas deferens, （87.375 ± 13.453） and （36.215 ± 11.429） U/g protein, respectively, which is significantly lower in spermatophore than insperm. Thirdly, compared with other reproductive tissues, the fumarate reductase activity is high in spermatophore and sperm, which are （19.945 ± 6.371） and （15.732 ± 2.434） U/mg protein, respectively, while the succinate dehydrogenase activity is undetectable. Therefore the ratio of SDH/FR is very low. Finally, histochemical studies of carbohydrate, protein, lipid, LDH, SDH and

关 键 词：红螯螯虾 雄性生殖系统 生化成分 同工酶 精子代谢 

分 类 号：Q959.223[生物学—动物学]