高产红曲色素菌株的复合诱变选育  

Compound Mutation Breeding of Monascus Pigment High-yielding Strain Monascus anka MACF6

作　　者：陈义光[1,2] 张晓蓉[1] 张丽[1] 彭德娇[1] 刘祝祥[1] 徐丽华[2] 

机构地区：[1]吉首大学生物资源与环境科学学院 [2]云南大学云南省微生物研究所,教育部微生物资源重点实验室,云南昆明650091

出　　处：《长江大学学报（自然科学版）》2005年第8期69-71,共3页Journal of Yangtze University(Natural Science Edition)

基　　金：国家自然科学基金资助项目(30270004);科技部基础研究重大项目前期研究专项资助项目(2002C001P).

摘　　要：以红曲霉MAC-8(Monascusanka)为出发菌株,经紫外线(UV)、亚硝基胍(NTG)和60Co多重复合诱变,选育到一株生产特性有明显改善的变异菌株MACF6。发酵试验表明,菌株MACF6发酵液色阶平均达到242U.mL-1,菌丝体色阶达1.09ⅹ104U.g-1,分别比出发菌株MAC-8提高了22.8%和19.8%。连续传代试验表明该菌株在遗传上是稳定的。A monascus pigment high-yielding mutant Monasuns anka MACF6 was screened from Monascus anka MAC-8 after being treated with UV,N-Methyl-N'-Nitro- N-Nitrosoguanidine(NTG)and γ-ray of^(60)Co.The average color value of three batches' fermented broth reached up to 242 U·mL^(-1),which was 22.8% higher than the original strain's.And the average color value of dry cell reached up to 1.09 X 10~4 U·g^(-1),which was 19.8% higher than the original strain's.The mutant's genetic stability was good after five generations.

关 键 词：红曲霉(Monascus anka) 红曲色素 复合诱变育种 

分 类 号：Q939.97[生物学—微生物学] TQ925.7[轻工技术与工程—发酵工程]