单侧颞叶皮质脑梗死大鼠空间认知能力与溶栓胶囊的干预效应(英文)  

Spatial cognitive capability of rats with lateral temporal cortical infarction and intervention effect of thrombolytic capsule

作　　者：董闽田[1] 管兴志[2] 匡培梓[3] 高扬[3] 管林初[3] 匡培根[2] 钱惠康[1] 

机构地区：[1]解放军第一七五医院神经内科,福建省漳州市363000 [2]解放军总医院神经内科,北京市100853 [3]中国科学院心理研究所三室,北京市100101

出　　处：《中国临床康复》2005年第32期223-225,共3页Chinese Journal of Clinical Rehabilitation

摘　　要：背景:颞叶皮质与人类和动物的学习记忆能力有关,它不仅接受听觉的投射纤维,还与许多空间认知相关的脑区有密切关系。目的:观察单侧颞叶皮质脑梗死大鼠经溶栓胶囊治疗后空间认识能力的改善情况。设计:完全随机对照实验。单位:解放军第一七五医院神经内科,解放军总医院神经内科及中国科学院心理研究所。材料:实验于1998-08/1999-02在中国科学院心理研究所脑功能研究室完成,选择SD大鼠28只,随机分为3组:脑梗死组10只,治疗组10只,假手术组8只。方法:立体定向光化学诱导制作脑梗死大鼠模型。水合氯醛麻醉,尾静脉注射玫瑰红溶液20mg/kg,左侧颞部切开头皮,暴露颞骨,光导纤维引导卤素灯冷光源,定向照射20min,面积2mm2。照射20min后治疗组腹腔注射溶栓胶囊生理盐水溶液(40g/L)200mg/kg,脑梗死组腹腔内注射等量生理盐水。假手术组尾静脉及腹腔注射生理盐水外,手术照射条件相同。主要观察指标:各组大鼠在Morris水迷宫中的训练成绩,包括:①反应时。②搜索策略(分为边缘式记1分,随机式记2分,趋向式记3分,直线式记4分)。结果:28只大鼠均进入结果分析。①各组大鼠平均反应时比较:治疗组和假手术组短于脑梗死组犤第1天:58,50,65s;第2天:24,27,46s;第3天:14,17,20s;第4天:11,9,15s,(P<0.01)犦,治疗组与假手术组基本接近(P>0.05)。②搜索策略评分:第1天可见假手术组及治疗组稍高于脑梗死组,但不稳定(F=1.167,P>0.05),第2天假手术组高于脑梗死组犤2.9,2.3分,(F=5.5,P<0.05)犦,但治疗组与脑梗死组基本接近犤2.6,2.3分,(F=3.34,P>0.05)犦,第3,4天治疗组均高于脑梗死组犤3.5,2.7分;3.7,3.3分,(F=8.92,14,P<0.01)犦。治疗组与假手术组基本接近(P>0.05)。结论:脑梗死早期给予溶栓胶囊治疗后,反应时明显缩短,搜索策略逐渐由边缘式和随机式过渡到趋向式和直线式,说明溶栓胶囊可改善脑缺血后的认知功能�BACKGROUND： Temporal cortex is related to human and animal＇s learning and memory capahility, it not only accepts the casting fibers of auditory sense, but alsn closely connects with many brain areas where are related to spatial cognition. OBJECTIVE： Tn observe the improving effect of thrombolytie capsule on spatial cognitive capability of rats with lateral temporal cortical infarction DESIGN： Completely randomized controlled study SETTING： Departmeni of Neurology, the 175^th Hospital of Chinese PLA; Department of Neurology, General Hospital of Chinese PLA; Institute of Psychotogy of Chinese Academy of Science MATERIALS： This experiment was carried nut at Brain Function Laboratory of Institute of Psychology of Chinese Academy of Science from August 1998 to February 1999, 28 SD rats were randomly divided into 3 groups, namely brain stroke group with 10 rats, treatment group with 10 rats and sham operation group with 8 rats. METHODS： Brain infarction model was established on SD rats by using three-dimensional directional pholoehemisiry induction. Rats were anaesthetized with hydrochloride and intravenously injected of rose pink solution in dosage of 20 mg/kg from tail vein. Then incision was made on the left temporal scalp to expose temporal bone, cold photosuurce with the guidance of optical fiber was used for directional illumination for 20 minutes in area of 2 mm^2. Twenty minutes later, rats in treatment group were given iniraperitoneal injection of thrombolytic capsule physiological saline of 40 g/L in dosage of 200 mg/kg, which replaced by the same volume of physiological saline in brain stroke group. Rats in sham-uperation group received the same operative lamination and injected with the same volume of physiological saline from tail vein and intraperitoneal cavity. MAIN OUTCOME MEASURES： Achievement of Morris water labyrinth training of all rats, including：①Response time.② Searching strategy （edge type scored 1, randomize type of 2, trend type of 3 and straight-line type of 4）. RESU

关 键 词：脑梗塞/药物疗法 胶囊 颞叶 空间知觉 迷宫学习 

分 类 号：R749.1[医药卫生—神经病学与精神病学]