东方巴贝斯虫的18S rRNA基因的扩增及系统发育研究  

Amplification of 18S rRNA Gene of Babesia orientalis and Study on Its Phylogenesis

作　　者：刘琴[1] 付媛[1] 周艳琴[2] 周丹娜[1] 江涛[1] 王金苹[1] 赵俊龙[1] 姚宝安[2] 

机构地区：[1]华中农业大学农业微生物国家重点实验室,武汉430070 [2]华中农业大学动物医学院,武汉430070

出　　处：《畜牧兽医学报》2005年第9期923-926,共4页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金资助(30070572)

摘　　要：利用真核生物18S rRNA基因的PCR通用引物对寄生于中国水牛的巴贝斯虫(已命名为东方巴贝斯虫-Babesia orientalis)基因组DNA进行扩增,得到其18S rRNA全基因片段,测序后blast分析表明该虫种属巴贝斯虫无疑。将该基因1 700 bp长片段序列与GenBank中15种已知巴贝斯虫的相应序列进行比较分析,建立系统发育树。结果表明,东方巴贝斯虫与南非未定种的巴贝斯虫亲缘关系最近,与羊巴贝斯虫亲缘关系较近,与牛巴贝斯虫、双芽巴贝斯虫的亲缘关系较远。这一结果说明水牛东方巴贝斯虫是一独立种。The complete 18S rRNA gene of Babesia orientalis parasited in buffalo in China was obtain by PCR. It was sequenced and then blasted. The result indicated that the parasite belonged to the genus Babesia . The 1 700 bp complete gene sequence was compared with 15 other Babesia sp. availabled in Gen-Bank. The data were analyzed and a phylogenetic tree was established. The result indicated that the parasite was close to Babesia sp. of South Africa and B. ovis, and the distant was far to B. bigemina and B. boris. This result indicated that it was no doubtful that it was a new species. It proved that the Buffalo babiesiosis was not mixinfected by B. bigemina and B. boris .

关 键 词：东方巴贝斯虫 水牛 PCR 18S RRNA 系统发育树 新种 

分 类 号：S852.723[农业科学—基础兽医学]