HSVECs血管生成模型和HUVECs血管生成模型的比较  

HSVECs and HUVECs angiogenesis model in vitro

作　　者：李斌[1] 刘宁[2] 张洪彬[1] 焦存仙[2] 

机构地区：[1]云南大学药学院云南大学生物制药创新人才培养基地,云南昆明650091 [2]云南省第一人民医院产科,云南昆明650032

出　　处：《云南大学学报（自然科学版）》2005年第5期457-460,共4页Journal of Yunnan University(Natural Sciences Edition)

基　　金：云南大学理(工)科校级科研项目资助(K1059069)

摘　　要：首次利用人隐静脉内皮细胞(HSVECs)在体外建立血管生成模型,并与脐静脉内皮细胞(HUVECs)模型进行比较.在不加入任何生长因子的条件下,在三维胶原系统中经过3 d的培养,HSVECs能形成毛细血管样网状结构.genistein是一种酪氨酸酶抑制剂,它能影响毛细血管网的形成.与HUVECs模型比较两者存在明显的差异性.HSVECs在体外三维模型中能形成一种变异型的毛细血管样网状结构.HSVECs angiogenesis model was first developed and compared with HUVECs angiogenesis model. HSVECs and HUVECs were separately sandwiched into the 3-D collagen gels to culture the angiogenesis model in vitro; the anti-angiogenic effects of genistein was assessed in this system. HSVECs have the capacity to form capillary-like structures in this model, the organization process was slower than HUVECs angiogenesis model. This new HSVEC angiogenesis model has been proved to be as a anti-angiogenic screening model.

关 键 词：内皮细胞 血管生成 体外模型 

分 类 号：R733.7[医药卫生—肿瘤]