荧光分析法测定痕量丁二酮  

Determination of Trace Diacetyl by Fluo-rescence

作　　者：王伦[1] 周运友[1] 朱昌青[1] 

机构地区：[1]安徽师范大学化学系

出　　处：《分析试验室》1996年第3期45-47,共3页Chinese Journal of Analysis Laboratory

摘　　要：本文报道了邻苯二胺与丁二酮在１ｍｏｌ／ＬＨ＿２ＳＯ＿４介质中反应生成２，３－二甲基喹喔啉。该化合物具有较强的荧光（λ＿（ｅｘ）／λ＿（ｅｍ）＝３４０ｎｍ／４９０ｎｍ），其荧光量子产率Φ＿Ｆ＝０．１０１。丁二酮浓度在４．０×１０￣（－７）～５．２×１０￣５ｍｏｌ／Ｌ范围内与转化成产物的相对荧光强度呈线性关系，相对标准偏差为１％，检出限为１．３×１０￣（－７）ｍｏｌ／Ｌ。考察了３０种物质表明，该法选择性好，用于实际啤酒样品中丁二酮的测定，结果令人满意。The reaction of o-phenylenediamine withdiacetyl is described,the product 2,3-dimethylquinoxaline(DMQX) has strong fluo-rescence(λ_(ex)/λ_(ex)=340nm/490nm).The fluo-rescent quantum yield Φ_f of DMQX in lmol/LH_2SO_4 is 0.101.There is a fine linear relation-ship between the fluorescent intensity of theproduct and the concentration of diacetyl in therange of 4. 0×10￣(-7)～5.2×10￣(-5)mol/L, The RS D is 1%. The detection limit is 1.3 ×10￣(-7)mol/L. This method has good selectivity andhas been applied to the determination of di-acetyl in beers with satisfactory results.

关 键 词：邻苯二胺 丁二酮 啤酒 荧光法 

分 类 号：TS262.5[轻工技术与工程—发酵工程] O657.3[轻工技术与工程—食品科学与工程]