针刺对备用背根节和脊髓NT-4表达的影响  

Influence of Acupunction on NT-4 Expression in Spared Root Ganglion and Spinal Cord

作　　者：龙双涟[1] 刘芬[1] 王廷华[1] 王特为[1] 柯青[1] 袁源[1] 

机构地区：[1]昆明医学院神经科学研究所

出　　处：《四川大学学报（医学版）》2005年第5期625-629,共5页Journal of Sichuan University(Medical Sciences)

基　　金：国家自然科学基金(批准号30260125)资助

摘　　要：目的观察NT-4在针刺部分去背根猫的L6背根节(手术侧和非手术侧)和脊髓表达的时空变化,以了解NT-4与针刺促进脊髓可塑性的关系。方法25只成年健康雄猫随机分为5组即正常组,备用根术(动物行单侧部分去背根手术,即切除一侧L1-L5、L7-S2背根节,保留L6为备用根)后7d组与14d组、针刺备用根术(动物行单侧部分去背根术后针刺)后7d组与14d组。动物处死后,取L6背根节及L3、L5、L6节段脊髓,用兔抗NT-4(1∶200)抗体行免疫组化ABC法染色。观察、计数并比较各组背根节、脊髓实验侧板层(L3、L5、L6)和背核(L3)中NT-4的分布及含量。结果针刺备用根7d组,针刺侧DRG内NT-4阳性神经元数较正常组和术后7d组减少(P<0.05),较相应非针刺侧多(P<0.05);针刺14d组,针刺侧DRG阳性神经元数比针刺7d组术后14d和同组非针刺侧增高(P<0.05),且恢复至正常水平。针刺7d,针刺侧脊髓L6板层NT-4阳性神经元数较术后7d组及正常组增加(P<0.01)。针刺14d时,L5、L6板层阳性神经元数较正常组及术后14d组相应节段均增加(P<0.01)。针刺后L3背核NT-4阳性神经元数无明显变化(P>0.05)。L5板层NT-4阳性神经元在针刺后14d时比针刺后7d时增多(P<0.05)。结论以上结果提示NT-4与针刺促进脊髓可塑性有关;针刺备用背根对非针刺侧DRGNT-4的表达亦有影响,提示非针刺侧背根节NT-4亦可能参与了脊髓可塑性和针刺促进脊髓可塑性。Objective To explore the changes of the expression of NT-4 in spared dorsal root ganglia （DRG,L6） on both the operation/Acup side and the nonoperation/non-Acup side as well as in the spinal lamina Ⅱ （L3, L5, L6） and Clarke＇ nucleus （L3） of the normal adult cats, partial dorsal rhizotomy cats, and Acup spared DRG cats so as to disclose the relation between NT-4 and the plasticity of spinal cord as well as the Acup promoting spinal cord plasticity. Methods Twenty-five adult cats were divided into 5 groups; normal control group; unilateral partial root rhizotomy 7 d and 14 d groups（unilateral L1-L5, L7-S2 DRG were transected, but L6 DRG was spared）; Acup spared DRG 7 d and 14 d groups （electro-needle stimulation was performed following unilateral partial root rhizotomy）. The cats survived for 7 or 14 days after operation respectively. Bilateral L6 dorsal root ganglia and L3, L5, L6 spinal cord of every group were made into 20 μm frozen sections. Then, sections were stained under the same condition using specific NT-4 （1：200） antibody by the immunohistochemistry ABC method. The distribution and the number of NT-4 immunoreactive neurons in bilateral spared DRG （L6） on the operation/Acup side and the nonoperation/Acup side as well as in the, spinal lamina Ⅱ （L3, L5, L6） and Clarke＇ nucleus （L3） of each cat were oberserved and counted. All data were analyzed by one-way ANOVA, SNK-q test and paired-t test. Results Partial dorsal root rhizotomy led to continuous declination of total NT-4 immunoreactive neurons in spared ganglia, till the 14 d, while Acup reversed this tendency and made NT-4 immunoreactive neurons decrease firstly and then approach to normal level till the 14 d after Acup. In addition, Acup increased NT-4 expression in L5, L6 spinal lamina Ⅱ.Conclusion The above finding indicate that NT-4 plays an important role in the mechanism by which Acup promotes spinal cord plasticity. Partial dorsal root rhizotomy and Acup spared DRG may exert effects on the expressio

关 键 词：部分去背根 针刺 免疫组化 脊髓可塑性 NT-4 

分 类 号：R245[医药卫生—针灸推拿学]