肿瘤患者HPRT基因的调查  

The Investigation of HPRT Gene in Malignant Tumor Patients

作　　者：周菊英[1] 王利利[1] 徐晓婷[1] 俞志英[1] 秦颂兵[1] 许昌韶[1] 

机构地区：[1]苏州大学附属第一医院肿瘤放射治疗科,江苏苏州215006

出　　处：《苏州大学学报（医学版）》2005年第4期611-612,646,共3页Suzhou University Journal of Medical Science

摘　　要：目的了解肿瘤患者与健康人群体细胞HPRT基因突变频率,探讨体细胞HPRT基因突变与肿瘤发生的关系。方法采用多核细胞法研究肿瘤患者外周血淋巴细胞HPRT基因位点的突变频率,并与正常人群进行对比。结果肿瘤患者外周血淋巴细胞转化率较正常对照组低(P<0.05);而淋巴细胞HPRT基因突变率较正常对照组高(P<0.05);不同种类癌症患者外周血HPRT基因突变频率无显著差异。结论HPRT基因突变频率分析可作为患癌风险评估的生物学标志。Objective To study the frequency of HPRT locus mutation in blood lymphocytes both in the healthy people and malignant tumor patients. Methods A simple, rapid and sensitive poly-nuclear cell assay was used to investigate the frequency of HPRT locus mutation. Results The frequency of HPRT in malignant tumor patients was higher than that in the healthy people, but there was no difference in different malignant patients. Conclusion The frequency of HPRT locus mutation may be expected to be a potential biological indicator for cancer risk assessment.

关 键 词：HPRT基因 突变频率 多核细胞法 

分 类 号：R730.231[医药卫生—肿瘤]