利凡诺对B16黑色素瘤细胞生长及MMP-2、MMP-9表达的影响  

Effect of ethacridine on the growth and the expression of MMP-2 and MMP-9 of B16 melanoma cells

作　　者：王莉[1] 陈平圣[1] 冯珍[1] 

机构地区：[1]东南大学基础医学院病理学与病理生理学系,江苏南京210009

出　　处：《东南大学学报（医学版）》2005年第5期289-293,共5页Journal of Southeast University(Medical Science Edition)

基　　金：国家自然科学基金资助项目(30470780);铁道部科技基金资助项目(J96Z069)

摘　　要：目的:观察利凡诺对体外培养的B16黑色素瘤细胞生长状态及基质金属蛋白酶-2(MMP-2)、基质金属蛋白酶-9(MMP-9)表达的影响,研究利凡诺的抗肿瘤转移作用。方法:用噻唑蓝(MTT)法检测利凡诺对B16黑色素瘤细胞的生长抑制率;酶联免疫吸附法检测细胞培养上清的MMP-2蛋白相对含量;免疫细胞化学染色检测MMP-2、MMP-9蛋白表达情况。结果:MTT法检测结果显示,不同浓度的利凡诺对细胞都有明显抑制并伴有时间及浓度依赖性;6.25、12.5μg.ml-1的利凡诺组在72 h与同时段对照组比有显著差异(P<0.05);利凡诺各药物组的MMP-2、MMP-9蛋白阳性表达随药物浓度的增加而减弱。结论:利凡诺可以抑制B16细胞的生长,并且具有潜在的抑制肿瘤侵袭和转移的作用。Objective To observe the effect of ethacridine on growth and expression of MMP-2 and MMP-9 of B16 melanoma cells. Methods After B16 cells were treated with various concentrations of ethacridine, inhibiting ratio of the growth was measured by MTT assay. The expression of MMP-2 protein was detected by ELISA. And then the expression of MMP-2, MMP-9 proteins was detected by immunocytochemistry. Results Proliferation of B16 melanoma cells in vitro was significantly inhibited（ P 〈 0.01 ）, showing a time-dependent and dose-dependent manner. MMP-2 secretion was significantly decreased in supernatant secreted by melanoma cells treated with higher concentrations of ethacridine（6.25,12.5μg·ml^-1, P 〈 0.05）. The increase of the expression of MMP-2 and MMP-9 proteins attenuated along with the increase of the concentration of ethacridine. Conclusion Ethacridine can markedly inhibit proliferation of B16 melanoma cells cultured in vitro and restrain their metastasis to some extent.

关 键 词：利凡诺 B16黑色素瘤细胞 基质金属蛋白酶 转移 

分 类 号：R739.5[医药卫生—肿瘤] R979.1[医药卫生—临床医学]