1,3-丙二醇氧化还原酶结构与功能关系的探讨  

Study the structure-function relationship of 1,3-propanediol Oxidoreductase

作　　者：林锦霞[1] 张光亚[1] 方柏山[1] 

机构地区：[1]华侨大学生物工程与技术系,福建泉州362021

出　　处：《生物信息学》2005年第3期101-103,共3页Chinese Journal of Bioinformatics

基　　金：国家自然科学基金资助项目(20276026);福建省科技计划项目(20031020)

摘　　要：用生物信息学方法对来源于klebsiella pneumonide的1,3-丙二醇氧化还原酶(即1,3-丙二醇氧化还原酶,PDOR)进行高级结构模建,并搜索其功能位点,以所得三维结构为对象,定位其铁信号结合位点、辅酶NADP大致位置以及可能的底物结合部位;在此基础上模拟PDOR活性部位,探讨该酶的构效关系。Based on the knowledge of bioinformafics, secondary and tertiary structure predictions of PDOR were performed with the biological macromolccule databases and software. The results showed that it contained a highly conserved iron- binding motif and belonged to a novel family of alcohol dehydregenases…… type Ⅲ alcohol dehydrogenases, Homology modeling and threading were used to model the tertiary structure. The result of homology modeling indicated that the conserved iron - binding motif formed a deft where the Fe^2 + ion may bound in, At the same time, The spatial relationship between iron, iron - binding site and NAD(P) was found, the possible mechanism of the active site was discussed.

关 键 词：1 3-丙二醇氧化还原酶 结构与功能 同源模建 活性部位 

分 类 号：Q554[生物学—生物化学]