过氧化物酶体增殖激活性受体γ(PPARγ)在肝癌组织中的差异性表达  

Enhanced expression of peroxisome proliferator-activated receptor γ in liver cancer

作　　者：郭鸣雷[1] 李锦军[1] 李宏年[1] 万大方[1] 顾健人[1] 

机构地区：[1]上海市肿瘤研究所生化室,编号200032

出　　处：《肿瘤》2005年第5期413-415,共3页Tumor

基　　金：国家重点基础研究发展规划项目(编号:20048704)

摘　　要：目的检测过氧化物酶体增殖激活性受体γ(PPARγ)在肝癌组织中有无差异性表达且有无突变。方法用半定量RT- PCR,Western blot,FISH,PCR-SSCP等方法进行鉴定。结果用半定量RT-PCR检测,有8/14为癌中高表达;Western blot 方法检测,9/15为癌中高表达。免疫组化显示PPARγ在所有的癌旁组织中定位于细胞浆中;而在5个癌组织中定位于细胞核中;突变热点PPARγ外显子3和5在34对肝癌标本中没有突变。结论过氧化物酶体增殖激活性受体γ在肝癌组织中有差异性表达并且在肝癌标本中没有突变。提示PPARγ可能与肝癌的发展有一定的相关性。Objective To investigate whether the PPARγ has high expression and mutation in liver cancer. Methods semi-quantitative RT-PCR,Western blot, FISH and PCR-SSCP method. Results PPARγ was over-expressed in matched HCC and non-cancerous tissues （8/14 by semi-quantitative RT-PCR and 9/15 by Western blot）. Immunochemical staining demonstrated that PPARγ was located in cytoplasmic in all non-cancerous liver in contrary to the nuclear localization in some of HCC cell. In addition, there was no mutation in the exon3 and exon5 of PPARγ in 34 paired HCC tissues. Conclusion It suggested that PPARγ may be involved with HCC development.

关 键 词：肝肿瘤 过氧化物酶体增殖激活性受体γ 基因表达 突变 

分 类 号：R735.7[医药卫生—肿瘤]