发根农杆菌K599对大豆、黄瓜和凤仙花活体感染生根的研究  

Hairy Root Induced by Wild-type Agrobacterium rhizogenes K599 in Soybean, Cucumber and Garden Balsam in vivo

作　　者：向太和[1] 王利琳[1] 庞基良[1] 陈敏[1] 许超[1] 

机构地区：[1]杭州师范学院生命科学学院,杭州310036

出　　处：《遗传》2005年第5期783-786,共4页Hereditas（Beijing)

基　　金：浙江省自然科学基金项目(编号:Y304083);浙江省教育厅科研项目(编号:20040069);杭州师范学院科研启动基金项目(编号:87)~~

摘　　要：利用野生型的发根农杆菌K599对属于不同科属的大豆、黄瓜和凤仙花进行活体感染实验,结果表明,发根农杆菌K599可使切割后的大豆、黄瓜和凤仙花子叶形成不定根,生根频率分别为100%、65%和91%。此外,发根农杆菌K599还可以使未进行创伤切割的黄瓜腋芽处形成不定根,生根频率为10%。根据发根农杆菌所具有的rolC基因序列,设计PCR引物,对再生的毛状根DNA进行PCR扩增,验证了再生的毛状根中含有发根农杆菌T-DNA序列。文章中获得的转基因根为进一步开展大豆、黄瓜的根结线虫病理以及凤仙花的矮化育种研究提供了前提材料。The plantlets of soybean, cucumber and garden balsam were inoculated by wild-type Agrobacterium rhizogenes K599, and hairy root was induced on inoculated sites in vivo. The frequencies of hairy root induction from wound cotyledons of soybean, cucumber and garden balsam were 100%, 65% and 91% respectively. Moreover, hairy root was induced from healthy cucumber axillary bud with frequency of 10%. PCR analysis of hairy root DNA was conducted using the primers from rolO gene. The PCR results showed that all hairy root lines contained T-DNA. The established system should be ideal for studying soybean and cucumber nematode and garden balsam breeding of flower dwarf architecture.

关 键 词：发根农杆菌 大豆 黄瓜 凤仙花 活体 生根 

分 类 号：S188[农业科学—农业基础科学]