宫颈染色体3p14杂合性缺失和微卫星不稳定性研究  

Study of loss of heterozygosity and microsatellite instability on chromosome 3p14 in cervical carcinoma

作　　者：刘炯[1] 孙小妹[1] 龚志强[2] 许小明[2] 王川来[1] 李燕坤 

机构地区：[1]北京航天总医院病理科,100076 [2]江西医学院病理教研室

出　　处：《中国现代医药杂志》2005年第4期31-34,共4页Modern Medicine Journal of China

摘　　要：目的探讨宫颈癌染色体3p14区域D3S1300、D3S1600位点微卫星不稳定性(microsatellite instability,MSl)及等位基因杂合性缺失(loss of heterozygosity,LOH)频率,为准确定位宫颈癌相关肿瘤抑制基因位点提供实验依据,并探讨LOH及MSI与宫颈癌临床分期、病理分级的相关性。方法选择3p14区域两个微卫星多态性位点,显微分离提取41例宫颈癌石蜡切片中的正常组织和肿瘤组织,经PCR扩增及聚丙烯酰胺凝胶电泳和硝酸银染色,进行LOH及MSI研究。结果41例样本中有25例至少存在一个位点的LOH,D3S1300位点LOH的频率为35%,D3S1600位点LOH的频率为28%。MSI发生频率相对较低,D3S1300的MSI频率为7.5%,D3S1600的MSI频率为12.8%。D3S1300、D3S1600位点LOH的发生率与临床分期、病理分级相关性有显著意义(P<0.05),而MSI与之无显著的差别(P>0.05)。结论3p14区域内D3S1300、D3S1600位点具有较高的LOH,提示这两个微卫星位点附近可能存在尚未克隆的与宫颈癌发生、发展相关的肿瘤抑制基因。宫颈癌染色体3p14区域LOH与临床分期、病理分级成正相关,提示检测该区域的LOH可作为病程进展及预后的重要参考指标。MSI在本研究中与宫颈癌临床分期、病理分级无明显相关。Objective To determine the frequency of LOH and MSI on 3p14 in cervical carcinoma （CC）, facilitate the isolation of the candidate tumor suppressor genes associated with CC and discuss the possible relations between LOH and MSI, clinical stage and clinic-pathology. Methods Analyzed LOH and MSI in 41 cases of CC using the polymerase chain reaction for amplification with 2 polymorphic microsatellite repeats on chromosome 3p14. Results 25 of CC cases showed at least one loci of LOH. The loci of D3S1600 was 28%, the higher frequency of D3S1300 was 35%. The occourence of LOH showed significant correlation in different clinical stage and clinic-pathology. The frequency of MSI were 7.5% and 12.8% respectively. But no significant difference in statistical analysis. Conclusion LOH on chromosome 3p14 are common in high grade of CC. These imply that LOH on 3p14 are the important determinants of clinical stage and clinicopathological characteris＇tics of patients with CC. Tumor suppressor gene that play a role in cervical carcinoma may be located on the 3p14, likely at or near D3S1300, D3S1600 loci.

关 键 词：宫颈癌 杂合性缺失 微卫星不稳定性 染色体3p14 肿瘤抑制基因 染色体3p14 微卫星不稳定性 基因杂合性缺失 宫颈癌发生 稳定性研究 聚丙烯酰胺凝胶电泳 肿瘤抑制基因 基因位点 临床分期 

分 类 号：R737.33[医药卫生—肿瘤]