流动注射光度法测定黄酒中蛋白质的含量和分子量分布  

Determination of Contents and Molecular Weights Distribution of Rice Wine Proteins by Flow Injection-Spectrophotometry

作　　者：林峰[1] 白少勇[1] 

机构地区：[1]浙江大学化学系,杭州310028

出　　处：《分析化学》2005年第10期1459-1461,共3页Chinese Journal of Analytical Chemistry

基　　金：2002年浙江省科技计划资助项目(No.2002C31059)

摘　　要：将流动注射技术引入Bradford蛋白质测定法,利用Sepadex G-100凝胶层析柱分离黄酒中的蛋白质,可直接测定蛋白质的含量和分子量分布.成品酒(熟酒)为5.32 g/L(6.86 g/L);高分子蛋白质(FW>25000)占10.52%(13.08%);中分子蛋白质(FW:25000～5000)占49.12%(54.12%);小分子蛋白质(FW<5000)占40.36%(32.80%),并得到黄酒中蛋白质含量与分子量分布的曲线谱图,可监控和预测黄酒的品质.Rice wine was directly introduced to a Sepadex G-100 gel chromatography column, and rice wine proteins were eluted from the column and assayed by bradford method connected a flow injection analysis. The contents and molecular weights distribution of rice wine proteins are as follows：for the bottled rice wine, the protein content is 5.32 g/L and the fractins of hgith molecular weights （ FW 〉 25000） , middle molecular weights （FW 25000-5000） and low molecular weights （FW 〈5000） are 10.52%, 49.12% and 40.36%, respectively. Compare with the bottled rice wine, those of mature rice wine are 6.86 g/L, 13.08% , 54.12% and 32.80%, respectively. The relation ship between contents and molecular weights distribution of rice wine protein was showed by an eluted curve. According to the eluted curve of rice wine protein, a series of profiles of rice wine proteins, was set up. It can be applied to evaluate and forecast the qualities of rice wine.

关 键 词：流动注射分析 Bradford蛋白质测定法 黄酒 高分子蛋白质 分子量分布 流动注射技术 蛋白质含量 光度法测定 小分子蛋白质 凝胶层析 

分 类 号：O657.3[理学—分析化学] TS262.4[理学—化学]