鸡肉中多种喹诺酮类兽药残留量的高效液相色谱测定研究  

Study on Determination of Four Quinolones in Chicken by HPLC

作　　者：占春瑞[1] 温志海[1] 卜延刚[1] 祝建新[1] 

机构地区：[1]江西出入境检验检疫局,江南南昌330002

出　　处：《食品科学》2005年第10期172-176,共5页Food Science

摘　　要：建立了一种测定鸡肉中氧氟沙星、诺氟沙星、环丙沙星、恩诺沙星兽药残留的高效液相色谱法。样品经乙腈提取,正己烷液-液分配去除蛋白和脂类物质,旋转蒸发除去乙腈,乙腈与水溶解残渣,过微孔滤膜,采用高效液相色谱荧光检测,外标法定量。氧氟沙星、诺氟沙星、环丙沙星、恩诺沙星在0.05～1.2μg/ml浓度范围内线性良好,相关系数为0.9997～0.9999。在0.05～1.0mg/kg浓度范围内,平均加标回收率在78.3%～101.8%之间,相对标准偏差为3.89%～12.43%。方法的最低检出限氧氟沙星为0.0037mg/kg、诺氟沙星为0.0037mg/kg、环丙沙星为0.0035mg/kg、恩诺沙星为0.0022mg/kg。该方法简便、快速,可满足鸡肉中多种喹诺酮类兽药残留量的检测。A reversed-phase high performance liquid chromatographic method has been developed for the simultaneous determination of four quinolone antimicrobial residues in chicken. Quinolone antimicrobial were extracted with acetonitrile, followed by hexane partitioning. After the solvent was vaporized with a rotary evaporator, Quinolone antimJcrobials were analyzed by HPLC-FLD and quantified by external standard method. The liner range was 0.05～ 1.21μg/ml, and the correlation coefficients were 0.9997～0.9999. The average recoveries of four quinolone antimicrobials in spiked chicken ranged from 78.3% to 101.8%, and the relative standard deviations were between 3.89% and 12.43%. The limit of detection was 0.0037mg/kg for ofloxacin, 0. 0037mg/kg for norfloxacin, 0.0035mg/kg for ciprofloxacin and 0.0022mg/kg for enrofloxacin. The results have indicated that this multiresidues developed were easier, faster. It has also demonstrated that this multiresidue analysis method could meet the requirements for simultaneous determination of many quinolone antimicrobials in chicken.

关 键 词：多残留分析 喹诺酮类 鸡肉 高效液相色谱法 

分 类 号：S859.796[农业科学—临床兽医学]