利用寡聚环糊精配基键合介质分离表没食子儿茶素没食子酸酯的新方法  

New Chromatographic Method for Separation of Epigallocatechin Gallate with Oligo-β-cyclodextrin Coupled Media

作　　者：徐军[1] 谭天伟[1] 

机构地区：[1]北京化工大学生命科学与技术学院,北京100029

出　　处：《化工进展》2005年第10期1172-1175,共4页Chemical Industry and Engineering Progress

基　　金：国家自然科学基金(No.29976004;20136020;50373003;20325622);北京市自然科学基金(No.2032013);高等教育博士点基金(No.20030010004)

摘　　要：研究了利用寡聚环糊精配基键合的高效凝胶介质分离纯化茶多酚中的表没食子儿茶素没食子酸酯的新方法。考察了不同种类的流动相对分离效果的影响。最终确定采用水/乙醇/乙腈为57/30/13(体积比)进行等梯度洗脱,一次色谱分离的收率和纯度分别为73%、98%,介质经过0.5mol/L的NaOH-水-30%乙酸简单再生后即可重复利用。A new chromatographic method for the separation of epigallocatechin gallate （EGCG） with oligo-β-cyclodextrin coupled media was investigated. Mobile phase used in the separation was optimized and the mixture of water/ethanol/acetonitrile （57/30/13, V/V） was finally chosen for the separation, with the recovery and purity of EGCG turning out to be 73%, 98%, respectively. The column could be regenerated with the simple sequence of 0. 5M NaOH-water-30% acetic acid solution and the separation ability of the media was restored.

关 键 词：环糊精 表没食子儿茶素没食子酸酯 茶多酚 

分 类 号：O658.1[理学—分析化学]