中西医结合治疗膀胱肿瘤的基础研究  

Basic Research of Integrative Therapy for Bladder Cancer

作　　者：王坚[1] 刘新光[2] 何承伟[2] 何惠娟[1] 吴平[1] 董忠[1] 伍秀东[1] 林立国[1] 

机构地区：[1]广东医学院附属医院,广东湛江524001 [2]广东医学院生物化学和分子生物学教研室,广东湛江524001

出　　处：《河北医学》2005年第11期964-967,共4页Hebei Medicine

摘　　要：目的:探讨转基因肿瘤疫苗与大蒜素联合治疗膀胱肿瘤的效果与机理。方法:脂质体转染法将B7.1基因导入鼠膀胱肿瘤(MBT-2)细胞。免疫荧光染色测定B7.1分子的表达。混合淋巴细胞培养MTT法和LDH释放法观察对免疫系统的影响。MTT法测定大蒜素对肿瘤细胞生长的影响。动物实验测定联合治疗效果。结果:B7.1转基因肿瘤疫苗能有效刺激淋巴细胞增殖。与大蒜素能产生协同抗癌效果。这种协同抗癌效果与淋巴细胞的特异性杀伤活性有关。结论:转基因肿瘤疫苗与大蒜素合用有更好的抗肿瘤效果。Objective： To investigate anti - tumor effect and possible mechanism of gene modified tumor vaccine combined with allicin for bladder tumor. Method： A carcinogen - induced munne bladder cancer cell line, MBT - 2, was transfected with lipofectin and B7 - 1 plasmid. The expression of B7 - 1 molecules on the cell surface was observed by immunofluorescence staining. The proliferation of spleen lymphocytes which were stimulated by MBT - 2 - B7 - 1 was determined by MTT assay. Cytotoxicity of lymphocyte was measured by LDH -release assay. Direct cytotoxicity of allicin in vitro was determined by MTT assay too. Anti - tumor activity of combination therapy was measured by animal experiment. Result： B7 - 1 gene modifled tumor cells can stimulate splenocyte growth apparently. This tumor cell combined with allicin have a synergic anti -tumor activity. This synergic anti -tumor effect may be related with specific cytotoxicity of lymphocyte. Conclusion： B7 - 1 gene modified tumor vaccine combined with allicin have a better antitumor activity.

关 键 词：转基因肿瘤疫苗 大蒜素 膀胱肿瘤 

分 类 号：R737.14[医药卫生—肿瘤]