表达7个高分子量谷蛋白亚基的小麦新种质创制、鉴定及分子细胞学分析  

Identification and Molecular Cytology Analysis of Novel Wheat Germplasm Expressing Seven High Molecular Weight Glutenin Subunits

作　　者：杨文杰[1] 舒焕麟[1] 颜泽洪[1] 刘登才[1] 周永红[1] 

机构地区：[1]四川农业大学小麦研究所,都江堰611830

出　　处：《Acta Genetica Sinica》2005年第11期1184-1190,共7页

基　　金：四川省教育厅重点资助项目(编号:2003A015);四川省科技厅麦类育种攻关项目(编号:2001-2)~~

摘　　要：报道了六倍体小黑麦和普通小麦杂交、回交获得的特异表达7个高分子量谷蛋白亚基的2个小麦新种质———NR98116-9-2和NR98116-9-3的创制、形态性状、染色体组成、遗传稳定性、抗条锈病鉴定以及HMW-GS组成的鉴定结果。这2个品系的植株形态为普通小麦型, Feulgen染色、GISH结合C-带综合鉴定表明:NR98116-9-2为3R /3D代换系,2n =42;NR98116-9-3为3R附加系,2n =44;减数分裂中期Ⅰ染色体配对正常,具有较高的遗传稳定性。接种鉴定表明:它们高抗小麦条锈病。种子全蛋白和高分子量谷蛋白选择性沉淀的SDS-PAGE分析表明:它们具有的7条高分子量谷蛋白亚基组成分别是1、14 +15、6r +8、4 +12和1、14 +15、6r+8、5 +10,其中包括2个Glu-B1位点。This paper describes the characterization of novel wheat lines NR98116-9-2 and NR98116-9-3 derived from crosses of wheat with hexaploid triticale and expressing seven different high molecular weight glutenin subunits by SDS-PAGE analysis. The results suggested that the two lines were similar to wheat in morphological characters and were stable genetically. The chromosome number of root tip cells of the two lines were 2n = 42 and 2n = 44 and the configuration of the pollen mother cells at metaphase Ⅰ were 21 Ⅱ and 22 Ⅱ ,respectively. The analysis of chromosome constitution by in situ hybridization and C-banding led to the conclusion that NR98116-9-2 was a 3R （3D） disomic substitution line and NR98116-9-3 was a 3R disomic addition line. The HMW-GS compositions were 1,14 ＋ 15,6r ＋8,4 ＋ 12 and 1,14 ＋ 15,6r ＋8,5 ＋ 10, respectively. It is very interesting that both lines may contain two Glu-B1 sites. The potential usefulness of the two germplasm that expressed seven different high molecular weight glutenin subunits in wheat quality improvement was discussed.

关 键 词：小黑麦 小麦 HMW-GS C-带 GISH 

分 类 号：S512.1[农业科学—作物学]