Generation and characterization of novel stromal specific antibodies  

作　　者：Sapna HALDER Debbie L.HARDIE Dagmar SCHEEL-TOELLNER Mike SALMON Christopher D.BUCKLEY 

机构地区：[1]Prostate Cancer Research Centre,Institute of Urology(3rd floor),University College London,67 Riding House Street,London W1W 7EJ,UK [2]Rheumatology Research Group,MRC Centre for Immune Regulation,Institute of Biomedical Research(3rd or 4th floor),University of Birmingham,Birmingham B152TT,UK

出　　处：《Cell Research》2005年第9期739-744,共6页细胞研究(英文版)

基　　金：This work was supported by Celltech R&D Ltd grant(No.RWJK08228).

摘　　要：Rheumatoid synovial fibroblasts were used as an immunogen to produce monoclonal antibodies selected for their reactivity with stromal cell antigens.Mice were immunised with low passage whole cell preparations and the subsequent hybridomas were screened by immunohistochemistry on rheumatoid synovium and tonsil sections.The aim was to identify those antibodies that recognised antigens that were restricted to stromal cells and were not expressed on CD45 positive leucocytes.A significant number of antibodies detected antigen that identified endothelial cells.These antibodies were further characterised to determine whether the vessels identified by these antibodies were vascular or lymphatic.From five fusions clones were identified with predominant reactivity with:1)fibroblasts and endothelial cells;or 2)broad stromal elements(fibroblast,endothelium,epithelium,follicular dendritic cells).A fibroblast-specific antibody that did not also identify vessels was not generated.Examples of each reactivity pattern are discussed.

关 键 词：STROMAL MESENCHYMAL non-haematopoietic RHEUMATOID FIBROBLASTS CD45 

分 类 号：R593[医药卫生—内科学]