胃癌患者血浆中p53基因突变的检测  

Detection of p53 gene mutation in the plasma of gastric cancer patients

作　　者：苏鹏程[1] 张连海[1] 万文徽[1] 任晖[1] 张桂国[1] 王怡[1] 邓国仁[1] 季加孚[1] 

机构地区：[1]北京大学临床肿瘤学院

出　　处：《北京大学学报（医学版）》2005年第5期523-526,共4页Journal of Peking University:Health Sciences

基　　金：北京大学肿瘤学院基金项目(0001;0422)资助~~

摘　　要：目的:检测并比较胃癌患者外周血浆、肿瘤组织以及癌旁正常黏膜中p53基因突变状况。方法:从96名胃癌患者的术前血浆、肿瘤组织及癌旁正常黏膜提取DNA,采用PCR、变性高效液相色谱分析(denaturinghighperformanceliquidchromatography,DHPLC)及测序技术,检测p53基因5～8外显子的突变,选择20例健康人的血浆作为正常对照。结果:96例胃癌患者肿瘤组织中,p53基因杂合性突变的检出率为19.8%(19/96),在其相应的外周血浆中,p53基因杂合性突变的检出率为5.2%(5/96),在p53基因突变阳性的外周血浆中,其对应的肿瘤组织均发现有同样的p53基因突变,在对应癌旁正常黏膜组织及20例健康人对照血浆中均未检出p53基因突变。肿瘤组织和外周血浆中p53基因突变与胃癌患者的临床病理特征无相关性。结论:胃癌患者血浆中可检测出p53基因突变,胃癌患者血浆中p53基因突变的分析有可能作为临床辅助诊断、疾病监测的指标之一。Objective： To investigated p53 gene mutation in plasma of gastric cancer patients. Methods： DNA extracted from plasma and matched tumor and tumor-adjacent normal tissues of 96 gastric cancer patients, and DNA from 20 healthy people were studied. Exons 5, 6, 7, and 8 of p53 were amplified by PCR. The mutation status was analyzed by denaturing high-performance liquid chromatography （ DHPLC ）, followed by direct sequencing of cases with aberrant chromatographic patterns. Results： Heterozygous mutations of p53 gene were detected in 19.9% （19/96） of primary tumor tissues and 5.2% （5/96） of corresponding plasma. All p53 gene mutations detected in plasma DNA consisted with mutations in the matched primary tumor samples. Neither the tumor-adjacent gastric mucosa tissues nor control plasma from healthy volunteers showed p53 gene mutation. No correlation was found between p53 mutation status and clinicopathological features of gastric cancer patients. Conclusion： p53 gene mutation in plasma can be detected in tissues and plasma of gastric cancer patients, which could be applied in screening and surveillance of this disease.

关 键 词：胃肿瘤 基因 P53 血浆 DNA突变分析 

分 类 号：R735.2[医药卫生—肿瘤]