替代FAO法测定无色书虱成虫对PH_3的抗药性  

AN ALTERNATIVE FAO METHOD FOR DERTERMININGLIPOSCELIS DECOLOR(PSOCOPTERA)TO PHOSOPHINE

作　　者：白青云[1] 曹阳[2] 

机构地区：[1]淮阴工学院农业工程与食品工程系,江苏淮安223001 [2]河南工业大学粮油食品学院,河南郑州450052

出　　处：《粮食储藏》2005年第5期11-13,56,共4页Grain Storage

基　　金：中国国家粮食局和澳大利亚国际农业研究中心合作项目(ACIARPHT98137)

摘　　要：在25±1℃,75%±2%RH条件下,采用FAO方法和延长熏蒸时间的替代FAO方法对无色书虱3个品系YNQJLd1、QLd-8和GXHZLd1的成虫进行毒力测定,分别得出PH3致死中浓度(LC50)。熏蒸20小时,YNQJLd1、QLd-8和GXHZLd1的成虫LC50值分别为0.0079、0.7127和1.7586 mg/L,抗性品系的抗性系数分别为90和222倍:熏蒸72小时,YNQJLd1、QLd-8和GXHZLd1的成虫LC50值分别为0.0024、0.06994和0.21962 mg/L,抗性品系的抗性系数创分别为29和92倍;熏蒸144小时,YNQJLd1、QLd-8和GXHZLd1的成虫LC50值分别为0.00126、0.02288和0.09917 mg/L,抗性品系的抗性系数分别为18和79倍。结论:无色书虱同一品系成虫随着熏蒸时间的延长,可在较低的磷化氢浓度下达到较好的熏蒸效果。本研究的测定为今后的书虱磷化氢抗性研究工作提供了基础数据,为实仓的磷化氢熏蒸试验提供了可靠的参考依据。The values of LC50 of aducts of 3 strains of L. decolor were determined through the method recommended by FAO and alternative FAO. Exposed 20h, the values of LC50 of adults of YNQJLdl,QLd - 8 and GXHZLdl were 0. 0097,0. 7127and 1. 7586mg/L respectively , the resistant factors（Rf）of resistant strains were 90 and 222 respectively;Exposed 72h, the values of LCs0 of adults of YNQJLdl,QLd- 8 and GXHXLdl were 0. 0024,0. 06994 and 0. 21962 mg/L respectively, the resistant factors（Rf）of resistant strains were 29 and 92 respectively; Exposed 144h, the values of LC50 of adults of YNQJLd1 QLd - 8 and GXHZLdl were 0. 00126,0. 02288 and 0. 09917 mg/L respectively, the resistant factors（Rf）of resistant strains were 18 and 79 respectively. The results showed that the LCs0 of adults of the same strain is detracted with extending exposed time, the insects can be controlled effectively at lower PH3 concentration. The results provided basic data for fumigation in practical warehouse.

关 键 词：无色书虱 磷化氢 抗药性 毒力测定 PH3 成虫 书虱 无色 抗药性 AO法 LC50值 熏蒸时间 

分 类 号：S379.5[农业科学—农产品加工]