饮食脂肪对小鼠脑聚集长链多不饱和脂肪酸的影响  

Effects of Dietary n-3 and n-6 Triglycerides on Long Chain Polyunsaturated Fatty Acid Accretion in Mouse Brain

作　　者：齐可民[1] 焦宏[1] 江载芳[1] 

机构地区：[1]首都医科大学附属北京儿童医院儿科研究所营养中心

出　　处：《首都医科大学学报》2005年第5期521-524,共4页Journal of Capital Medical University

基　　金：北京市留学归国人员择优资助优秀项目;国家自然科学基金面上项目(青年基金)(30200303)资助课题

摘　　要：目的探讨高脂饮食状态下不同脂肪酸种类对脑聚集二十二碳六烯酸(DHA)和花生四烯酸(AA)的影响。方法采用C57BL/6J雌性小鼠,在成年期和幼年期分别给予不同种类高脂饲料(18%脂肪,供能比为36%)喂养,分为高脂豆油饲料、高脂鱼油饲料和高脂豆油∶鱼油(5∶1)混合饲料组,以正常饲料(6%脂肪来自豆油,供能比为12%)为对照,时间为4个月。然后采用气相色谱法对小鼠脑组织脂肪酸进行测定。结果4组不同饲料对脑组织中DHA和AA水平的影响在成年期和幼年期小鼠之间无差异。与高脂豆油饲料组相比,高脂鱼油饲料组小鼠脑组织中DHA的含量无显著变化,但AA及其前体亚油酸(LA)含量均显著降低,导致DHA/AA比值升高;高脂豆油∶鱼油混合饲料组小鼠脑组织中DHA、AA及其前体LA含量均无显著变化,但DHA/AA比值升高。结论饮食中鱼油n-3脂肪的摄入增加不影响脑DHA水平,但减少了AA的聚集,使得DHA/AA比值升高。Objective In this study the effects of dietary n-3 and n-6 fatty acids on brain accretion of docosahexaenoic acid （DHA） and arachidonic acids （AA） were investigated. Methods C57BL/6J female adult mice were fed three different high-fat （18 % fat by weight supplying 36 % of total energy） diets： soy oil, fish oil and soy：fish oil （5：1） respectively with normal chow diet （6% of fat as soy oil supplying 12% of total energy） as control, for a period of 4 months. Also some mice were mated for breeding new mice with high-fat diets being started 1 month before mating and ended at 2 months following mouse birth. Brain fatty acids were analyzed by gas chromatography and expressed as percent（ % ） of total fatty acids. Results No differences were found in the effects of dietary fat on DHA and AA levels in brain between young mice and adult mice. Compared to soy oil-fed mice, AA accretion in brain was decreased with no changes in DHA accretion leading to increased DHA/AA ratio in fish oil-fed mice, whereas no changes in both DHA and AA accretion were found but DHA/AA ratio was increased in soy： fish oil-fed mice. Conclusion Increased intake of fish oil n-3 triglycerides does not enhance DHA accretion but increases DHA/AA ratio in mouse brain.

关 键 词：N-3脂肪酸 n-6脂肪酸 脑 小鼠 

分 类 号：R151.2[医药卫生—营养与食品卫生学]