中国辽宁汉族人群D12S1686位点基因频率分析  

The analysis of gene frequency of D12S1686 in Han ethnic population of Liaoning,China

作　　者：王岳平[1] 麻宏伟[1] 李祁伟[2] 卢瑶[1] 姜俊[1] 王旸[1] 马瑞雪[2] 吉士俊[2] 

机构地区：[1]中国医科大学第二临床学院遗传研究室 [2]中国医科大学第二临床学院小儿骨科,沈阳110004

出　　处：《中国优生与遗传杂志》2005年第10期13-15,共3页Chinese Journal of Birth Health & Heredity

基　　金：国家自然科学基金(39970762);辽宁省自然科学基金(972248)~~

摘　　要：目的获得并研究中国辽宁汉族人群D12S1686位点的基因频率.方法应用聚合酶链反应和变性聚丙烯酰胺凝胶电泳技术检测188名无关的中国辽宁汉族人D12S1686位点的基因型,对此位点进行基因频率分析.结果在中国辽宁汉族人群D12S1686位点处共检测到16个等位基因,59个基因型,基因频率在0.003～0.245之间、杂合度为0.855、个体识别能力为0.904、多态信息含量为0.812,与高加索人群(白种人)的基因频率差异显著(P＜0.001).结论D12S1686位点具有较高的杂合度和多态信息含量,在基因连锁分析和群体遗传学研究中具有重要的应用价值.Objective：To acquire and investigate the gene frequency of D12S1686 in Han ethnic population of Liaoning, China. Methods The genotypes of 188 unrelated Han ethnic individuals in Liaoning were detected by the techniques of polymerase chain reaction （PCR） and denaturing polyacrylarnide gel electrophoresis, and the gene frequency in D12S1686 was subsequently analyzed. Results：There are 16 alleles and 59 genotypes detected in D12S1686 among 188 individuals of Han ethnic population of Liaoning, China with allele frequency from 0.003 to 0.245, Het of 0. 855, DP of 0.904, PIC of 0.813, which is significantly different from that of Caucasoid population （x^2 = 48.456, P 〈 0.001 ）. Conclusion： D12S1686 is such a valuable genetic marker that it can be used to gene linkage analysis and population genetics research.

关 键 词：汉族 D12S1686位点 基因频率 

分 类 号：Q987[生物学—遗传学]