IL-8与胃癌细胞SGC7901 MMP-2和MMP-9表达的关系  

Relationship of MMP-2 and MMP-9 expression of SGC7901 with IL-8 in vitro

作　　者：林晓[1] 王娅兰[1] 

机构地区：[1]重庆医科大学基础医学院病理教研室,400016

出　　处：《重庆医学》2005年第11期1657-1658,1661,共3页Chongqing medicine

摘　　要：目的探讨趋化因子白介素-8(IL-8)与胃癌细胞SGC7901细胞胶原酶MMP-2和MMP-9表达的关系。方法体外细胞培养,MTT法及免疫组化方法检测细胞增殖率及MMP-2,MMP-9蛋白表达。结果IL-8处理组SGC7901细胞MMP-2表达较对照组(无IL-8)比较,具有显著增强(P<0.05),而MMP-9无明显差异。结论IL-8能促进细胞SGC7901 MMP-2表达,而对MMP-9无明显影响。Objective To study the effect of expression of MMP-2 and MMP-9 in gastric cancer cell line SGC7901 with IL-8 in vitro. Methods The proliferative activity was determined by MTT assay. MMP-2 and MMP-9 expression was detected by immunohistochemistry. Results Cultured with IL-8 for 72h,MMP-2 expression of SGC7901 was more than that of control group. （P〈0.05）. Conclusion The data suggest that IL-8 can increase expression of MMP-2 protein of SGC7901.

关 键 词：SGC7901 白介素-8 MMP-2 MMP-9 细胞培养 免疫组织化学 

分 类 号：R735.2[医药卫生—肿瘤]