PTEN基因转染对人膀胱癌细胞BIU-87凋亡的影响  

Effects of PTEN gene transfection on apoptosis of human bladder cancer cell lines BIU-87

作　　者：张建华[1] 杨为民[1] 周四维[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院泌尿外科,湖北武汉430030

出　　处：《医学研究生学报》2005年第11期969-971,i0007,共4页Journal of Medical Postgraduates

基　　金：武汉市科技攻关课题资助项目(批准号:20036004056)

摘　　要：目的:研究外源性抑癌基因PTEN转染对人膀胱癌细胞系B IU-87凋亡的影响,探索膀胱癌基因治疗的新途径。方法:将携有PTEN基因的真核表达载体体外转染B IU-87细胞,筛选稳定转染的细胞并扩增培养,用逆转录聚合酶链反应法(RT-PCR)检测PTEN的表达情况,应用细胞原位凋亡检测试剂盒和流式细胞仪分别研究PTEN基因转染前、后膀胱癌细胞凋亡的变化。结果:转染PTEN基因的膀胱癌细胞系B IU-87中PTEN mRNA能稳定表达,肿瘤细胞凋亡明显增多,与对照组比较有显著性差异。结论:转染外源性PTEN基因能显著诱导膀胱癌细胞凋亡,从而达到抑制肿瘤发生、发展的目的。Objective：To study the effects of anti-oncogene PTEN transfection on apoptosis of human bladder cancer cell lines, BIU-87, and to bring a new method of gene therapy for bladder cancer. Methods：A eukaryotic expression vector containing PTEN was transfected into BIU-87,and positive cell clones were selected and amplified. Expression of PTEN was detected by RT-PCR. Apoptosis of BIU-87 were measured before and after transfection by in situ cell apoptosis detection kit and flow cytometry. Results ： PTEN transfected BIU-87 could steadily express PTEN mRNA, and cell apoptosis significantly increased compared with control groups. Conelusion：Transfection of PTEN might induce apoptosis of bladder cancer cells so as to inhibit the occurrence and development of tumor.

关 键 词：PTEN基因 转染 膀胱癌 基因治疗 

分 类 号：R737.14[医药卫生—肿瘤]