大鼠心肌缺血预适应延迟相的差异蛋白质的分离及鉴定  

Seperation and Identification of Differential Proteins of Late Phase of Myocardial Ischemic Preconditioning in Rats

作　　者：王尧玲[1] 王建设[1] 刘双[1] 袁灿[1] 吕青兰[1] 刘梅冬[1] 刘可[1] 肖献忠[1] 

机构地区：[1]中南大学湘雅医学院病理生理学教研室,湖南省长沙市410078

出　　处：《中国动脉硬化杂志》2005年第4期456-460,共5页Chinese Journal of Arteriosclerosis

摘　　要：目的采用蛋白质组学技术探讨缺血预适应心肌内源性保护的分子机制。方法应用双向凝胶电泳技术分离假手术组和心肌缺血预适应延迟相组大鼠的心肌总蛋白质。结果在假手术组的双向凝胶电泳图谱上展示704±27个蛋白质点,而在心肌缺血预适应延迟相组展示778±35个蛋白质点。经凝胶图像分析找到的差异蛋白质23个,基质辅助激光解析—电离飞行时间质谱对差异蛋白进行鉴定,共鉴定出12个蛋白质。这些蛋白质包括应激反应蛋白、代谢相关蛋白、信号调节蛋白、结构蛋白等。结论这些差异蛋白可能通过其分子伴侣功能、清除自由基、改善能量代谢等发挥心肌缺血预适应延迟相的心肌保护作用。Aim To search for global protein changes in ischemic preconditioning （IP） using proteomic technology. Methods The SD rats were divided into two groups including IP group and sham groups. The left ventricle tissues of preconditioned and sham groups were directly sampled for proteomic analysis. Results Analysis of two dimensional electrophoresis （2-DE） showed： 704 ± 27 protein spots were seen in Sham ventricle gels and 778± 35 protein spots in IP ventricle gels. Differential analysis using the Studem＇s t-test （P 〈 0.05） showed that the expression of 23 protein spots changed, 12 protein spots were identified by mass spectrometry. Cjonclusions Myocardial IP resulted in the changes of protein expression profiles in the myocardium. The differential proteins might function as molecular chaperone, decrease free radical and promote the energy metabolism of myocardium to confer cardioprotection.

关 键 词：病理学与病理生理学 缺血预适应延迟相的差异蛋白质分析 双向凝胶电泳 缺血预适应 蛋白质组 质谱 

分 类 号：R363[医药卫生—病理学]