NGF、BDNF和NT3在AD大鼠海马中的分布及表达变化  

Distribution and Change of NGF,BDNF and NT_3 in the Hippocampus of Rat with AD

作　　者：张卓[1] 王廷华[2] 朱榆红[1] 冯忠堂[2] 孙冰[1] 胡艳丽[2] 

机构地区：[1]昆明医学院第二附属医院神经内科,昆明650031 [2]昆明医学院神经科学研究所

出　　处：《四川大学学报（医学版）》2005年第6期789-791,833,共4页Journal of Sichuan University(Medical Sciences)

基　　金：国家自然科学基金(批准号30260112)资助

摘　　要：目的观察NGF、BDNF和NT3在A lzhe im er d isease(AD)大鼠海马中的分布及其表达变化。方法采用海马注射Aβ淀粉蛋白的方法建立AD模型。10 d后对大鼠进行灌注,取脑,冰冻切片,用NGF、BNDF和NT3抗体行免疫组织化学染色。对海马恒定视野内NGF、BDNF和NT3的阳性细胞进行记数并进行统计学分析。结果AD组海马中的NGF阳性细胞较正常组显著增多(P<0.01),且染色增强。BDNF阳性细胞较正常组明显减少(P<0.01),染色强度减弱。而NT3的阳性细胞数及染色强度与正常组比较差异均没有统计学意义(P>0.05)。结论NGF、BDNF和NT3在AD的海马中发生了不同的变化,提示NGF、BDNF和NT3在AD中发挥了不同的作用,尤其是BDNF阳性细胞的减少可能与AD的神经功能减退有关。Objective To investigate the distribution and change of NGF, BDNF and NTs in hippocampus of rat with Alzheimer disease（AD） by immunohistochemistry. Methods AD model was established by injecting β amyloid protein into the hippocampus of rat. The rats were killed ten days after injection. The hippocampus sections were made coronally on a freezing microtome, Brain sections were processed by immunohistochemical procedure with molocloned antibodies against NGF, BDNF and NTs, The number of positive neurons of NGF, BDNF and NTs was counted and analyzed statistically, Results It was found that the number of NGF positive neurons increased and the immunostaining intensity became stronger as compared with the control （P〈0.01）, The number of BDNF positive neurons decreased and the immunostaining intensity weakened （P〈0.01）. There was no change in the number and immunostaining intensity of NTspositive neurons （P〉0.05）, Conclusion Our results show that NGF, BDNF, NTs have experienced different changes in hippoeampus of rat with AD, suggesting that they play different roles in the course of AD and bear relation to the physiological function of cholinergic neurons in hippocampus of rat with AD. In particular, BDNFexerts crucial effect on the degeneration of neuronal function in the hippocampus of rat with AD.

关 键 词：神经生长因子 脑源性神经生长因子 神经营养因子3 ALZHEIMER 海马 SD大鼠 

分 类 号：R749.1[医药卫生—神经病学与精神病学]