三种MAGE基因在广西肝细胞癌中的表达  

Expression of three cancer-testis antigens, MAGE (melanoma antigen), in primary hepatocellular carcinoma in Guangxi area

作　　者：谢小薰[1] 何少健[1] 肖绍文[2] 范蓉[1] 莫发荣[1] 罗国容[1] 

机构地区：[1]广西医科大学基础医学院组织学与胚胎学教研室,南宁530021 [2]广西医科大学第一附属医院外科,南宁530021

出　　处：《肿瘤》2005年第6期585-588,共4页Tumor

基　　金：国家自然科学基金(编号:30360026);广西壮族自治区教育厅科学基金(编号:桂科自0229032)

摘　　要：目的检测癌-睾丸抗原(CTA)基因MAGE-1、-3和-4在广西地区肝细胞癌(HCC)中的表达,探讨CTA作为疫苗对HCC治疗的可行性。方法用逆转录聚合酶链反应(RT-PCR)法对25例广西HCC患者癌组织中MAGE-1、-3和-4基因mRNA的表达进行检测；随机选择MAGE-1、-3和-4阳性的RT-PCR产物各2例进行DNA序列测定。结果 MAGE-1和-3表达率均为40％(10／25),MAGE-4表达率为16％(4／25)。DNA测序结果表明RT-PCR产物为MAGE-1、-3和-4基因。统计学分析显示MAGE-1、-3和-4基因的表达与HCC组织分化程度及临床分期无相关性(P＞0．05),与HBV感染有相关性(P＜0．05)；此外,MAGE-1和-3的表达与肿瘤大小及MAGE-3和-4与AFP水平均存在相关性(P＜0．05)。在所检测的标本中,56％的标本至少表达上述1种MAGE,24％的标本至少表达2种MAGE；16％的标本表达3种MAGE。结论MAGE-1和-3在广西HCC中有一定频率的表达,能否用于HCC的免疫治疗值得深入探讨。Objective To investigate the expressions of cancer-testis antigens（CTA）, MAGE-1, -3 and -4, in primary hepatocellular carcinoma （HCC） of Guangxi area, and to explore the feasibility of CTA as a vaccine for immunotherapy of HCC. Methods Twenty-five HCC specimens were analyzed for the expressions of MAGE-1, -3 and 4 by RT-PCR. Two samples selected randomly from each MAGE PCR positive products were sequenced. Results Expressions of MAGE 1, -3, and -4 were 40% （10/ 25）, 40 % （10/25） and 16 % （4/25）, respectively. DNA sequencing results further confirmed that RT-PCR products were truly MAGE cDNA. In addition, statistic analysis showed that MAGE expressions had no correlation with tumor histological differentiation or clinic stage （ P 〈 0.05）, but had correlation with HBV infection （ P 〈0.05）. Meanwhile, the expression of MAGE-1 and -3 had correlation with tumor size, so had the expression of MAGE 3 and -4 with AFP level （ P 〈0.05）. Furthermore, 56% of HCC samples tested were expressed in at least one MAGE, 24% in at least two MAGEs, 16% in all the three MAGEs. Conclusion There are frequent expressions of MAGE-1 and -3 in HCC of Guangxi area, but whether they can apply to the immunotherapy of HCC needs to be further studied.

关 键 词：肝肿瘤 癌 肝细胞 黑色素瘤抗原 基因表达 逆转录聚合酶链反应 

分 类 号：R735.7[医药卫生—肿瘤]