HIV-1包膜糖蛋白gp120对大鼠海马脑片电生理特性的影响  

Effect of HIV-1 gp120 on electrophysiological characteristics in hippocampal slices of rats

作　　者：董军[1] 李传行[2] 陆大祥[1] 张穗梅[1] 

机构地区：[1]暨南大学医学院病理生理教研室,广东广州510632 [2]中山大学肿瘤防治中心,广东广州510060

出　　处：《基础医学与临床》2005年第11期1028-1032,共5页Basic and Clinical Medicine

基　　金：国家中药管理局择优项目(国中医药科2003LHR13号);广东省医学科研基金(A2004327);广东省自然科学基金(049213);暨南大学病理生理重点实验室开放基金(51205026)

摘　　要：目的探讨人类免疫缺陷病毒I型(HIV-1)的包膜糖蛋白gp120对大鼠海马脑片CA1区神经元电生理特性及突触传递的影响。方法用盲法全细胞记录技术,观察gp120对大鼠海马脑片CA1区神经元电生理特性及对高频电刺激Schaffer侧支引起的鼠海马长时程增强效应(LTP)的影响。结果①在电流钳,gp120可使终末去极化电流激发快速动作电位的数目增加;②在电压钳,gp120对大鼠海马CA1区神经元的全细胞电流无明显作用;③将gp120(100 pmol/L)与海马脑片共孵育1h后,在钳制电压为-60 mV时,发现HFS后海马CA1区的兴奋性突触后电流(EPSC)显著减小,LTP的强度减少到(108.5±8.0)%(n=11,P<0.01)。结论gp120可使海马神经元的兴奋性增加,并可能通过抑制海马CA1区的LTP诱发参与艾滋病痴呆(HIV-1 associated dementia,HAD)的病理生理过程。Objective To understand the effect of gp120 on electrophysiologocal characteristics and synaptic transmission in the CA1 region of hippocampal slices of rats. Methods We recorded the excitatory postsynaptic current （EPSC） by using ＇ blind＇ whole-cell recording techniques to investigate the effects of gp120 on electrophysiologocal characteristic and long-term potentiation in hippocampus in vitro of rats. Results The results showed as follows：Under current clamp, gp120 increased numbers of the fast action potentials elicited by a terminal depolarizing current. Under voltage clamp, gp120 didn＇t affect the whole cell current in CA1 neurons, while incubating hippocampal slices with 100pmol/L gp120 for lh, at holding potentials of -60 mV, HIV-1 gp120 could inhibit the EPSC and LTP of CA1 （n = 11, P 〈 0.01）. Conclusion gp120 can increase neurons excitability. This inhibition of LTP by gp120 may contribute to the pathogenesis of HIV-1 associated dementia（HAD）.

关 键 词：人类免疫缺陷病毒Ⅰ型 GP120 兴奋性突触后电流 长时程增强 海马脑片 

分 类 号：R749.1[医药卫生—神经病学与精神病学]