不同锌营养状态对小鼠淋巴细胞相关免疫功能的影响  

Effect of zinc on immune function in mice spleen lymphocytes

作　　者：陈竞[1] 曲宁[1] 夏弈明[1] 程桂芳[2] 

机构地区：[1]中国疾病预防控制中心营养与食品安全所,北京100050 [2]中国医学科学院药物研究所

出　　处：《卫生研究》2005年第6期710-713,共4页Journal of Hygiene Research

摘　　要：目的旨在观察不同锌营养状况对小鼠淋巴细胞相关免疫功能的影响。方法在锌耗竭期,分低锌组(DC)(饲料含锌5.2mgkg)、对饲组(PZ)和正常锌组(饲料含锌25.6mgkg)。继后的锌补充期,分低锌组(DC)、低锌正常组(DZNZ)和对饲自由组(PZNZ)。动态观察免疫功能相关指标如脾细胞数,淋巴细胞转化率,和刀豆蛋白A刺激的IL2的分泌情况。结果缺锌70天后可明显降低脾细胞数目,补锌22天后增加。缺锌可抑制脾T细胞的增殖反应,补锌后也有所上升。低锌组小鼠脾细胞分泌的IL2下降,补锌后可逐步恢复。结论锌可影响免疫系统的功能。IL2的变化较明显。Objective In order to study the effects of different levels of zinc nutrition status on the immune function of mice spleen lymphocytes. Methods The experiment included two periods： zinc depleted period and repletion period. In the depleted period, mice were divided into 3 dose groups： zinc deficient group （ZD, 5.2mg/kg）, zinc pair-fed group （PZ） and normal zinc group （NZ,25.6mg/kg）. In the repletion period, mice were divided into 3 dose groups： ZD group, DZ-NZ group and PZ-NZ group. It was measured some biomarkers of immunity to assess the zinc nutritional status, including lymphocytes amount, lymphocytes proliferation index and IL-2 activity. Results The lymphocytes counts of ZD group spleen showed significant decline at seventy days fed zinc deficiency diets. The counts of ZD-NZ group were increased after 22 days of taking normal zinc diet. Zinc deficiency could suppressed the proliferation of the spleen lymphocytes. The spleen lymphocytes proliferation of the ZD-NZ group showed a moderate but non-significant increase compared with the ZD group. Zinc deficiency could decrease IL-2 production. The production of IL-2 of ZD group was lower than those of ZP （ P 〈 0.05 ）, IL-2 production increased following zinc supplement. Conclusion It is found that zinc status can affect the immune function. Production of IL-2 in spleen lymphocyte is affected evidently by zinc.

关 键 词：锌 免疫 IL-2 

分 类 号：R151.3[医药卫生—营养与食品卫生学] Q581[医药卫生—公共卫生与预防医学]