三七地下部分皂甙成分的HPLC比较研究  

HPLC Comparative Analysis of Ginsenoside Saponins in Different Underground Parts of Panax notoginseng

作　　者：王东[1] 李海舟[1] 陈可可[1] 张颖君[1] 杨崇仁[1] 

机构地区：[1]中国科学院昆明植物研究所植物化学与西部植物资源持续利用国家重点实验室,云南昆明650204

出　　处：《云南植物研究》2005年第6期685-690,694,共7页Acta Botanica Yunnanica

基　　金：国家自然科学基金(30472156)

摘　　要：应用HPLC定量分析方法,对三七(Panax notoginseng)地下部位的皂甙成分进行分析,通过比较人参皂甙Rg1,Rb1,Re,Rd和三七皂甙R1等5种主要皂甙成分和总皂甙的含量变化,探讨不同部位和组织中皂甙成分的分布规律。结果表明在三七药材的不同商品等级中,人参皂甙Rg1和Rb1的含量以主根60头为最高,5个主要皂甙的总含量也明显高于其他的等级;根茎的生物产量只为全根的18%,皂甙含量占25%以上;主根和根茎中韧皮部的生物产量和总皂甙的含量均高于木质部;二年生三七的生物产量及皂甙含量均较三年生三七低得多。不同表型三七的皂甙组成也有区别。In order to elucidate the distribution ride of saponins in Panax notoginseng, the contents of ginsenosides Rg1, Re, Rb1 , Rd and notoginsenoside R1 in different underground parts of notoginseng were determined by HPLC analysis. The results indicated that the contents of ginsenosides Rg1 and Rb1 together with total content of ginsenosides Rg1, Rb1, Rd, Re, notoginsenoside R1 in the taproot ＂60 Tou＂ （viz. 60 taproots per 500 g） are highest among all commercial grades of P. notoginseng. The rhizome provided more than 25% saponins, though its biomass is only about 18% of the underground parts. The levels of biomass and saponins of phloem in both taproot and rhizome are significantly higher than those of xylem. The biomass and saponin levels of two years old roots are markedly lower than those of three years old one. The comparative analyses were carded out on P, notoginseng of different stem colors.

关 键 词：三七 皂甙 人参皂甙 三七皂甙 高压液相色谱 

分 类 号：R284[医药卫生—中药学]