培养条件对里氏木霉306菌体形态和t-PA生物合成的影响  

Influence of culture conditions on mycelium morphology and t-PA production by Trichoderma reesei 306

作　　者：冮洁 杜连祥[1] 路福平[1] 姚蕾[2] 于海龙[2] 江成英[2] 曹畅[2] 

机构地区：[1]天津科技大学食品科学与生物工程学院,天津300222 [2]齐齐哈尔大学生命科学与工程学院,齐齐哈尔161006

出　　处：《工业微生物》2005年第4期9-14,共6页Industrial Microbiology

基　　金：黑龙江省自然科学基金资助项目(No.D0352)

摘　　要：里氏木霉(Trichoderma reesei)306是能够生物合成组织型纤溶酶原激活剂(t-PA)的基因工程菌株。在对其液态深层培养时,发现随培养条件和培养时间的变化,其菌体能以松散和菌丝球的两种形态存在。菌体形态和t-PA的产生密切相关。培养基中无机盐和表面活性剂的种类和添加量以及接种量和pH等培养条件是影响里氏木霉306菌体形态和t-PA合成的主要因素。在液态深层培养过程中,菌体以松散的菌丝体形态生长,形成纸浆状发酵液,利于t-PA的合成。Trichoderma reesei 306 was a recombinant strain that can produce tissue-type plasminogen activator（t-PA ） . During submerged cultivation , the morphology of the filamentous fungal was usually two types： pellet and dispersed form. The morphology had relationship with t-PA production . The kind and concentration of metal ions and surfactants in fermentation medium , the inoculum size and pH were the main factors influencing the t-PA biosynthesis . In submerged culture course , the dispersed mycelium in growth phase forming pulpy fermentation broth was advantageous to t-PA production.

关 键 词：丝状真菌 里氏木霉 组织型纤溶酶原激活剂 菌体形态 

分 类 号：TQ920.1[轻工技术与工程—发酵工程] TQ465.5