重组人源性抗HBsAg Fab抗体的肽质量图谱分析  

Analysis of Peptides Mass Spectrum of Recombinant Humanized Anti-HBsAg Fab

作　　者：邓宁[1] 向军俭[1] 唐勇[1] 王宏[1] 

机构地区：[1]暨南大学生命科学技术学院分子免疫学与抗体工程实验室,广州510632

出　　处：《中国生物工程杂志》2005年第B04期224-227,共4页China Biotechnology

基　　金：国家自然科学基金资助项目(30471607)广州市科技局重点资助项目(2003J1-CO171)

摘　　要：重组人源性抗HBsAg Fab抗体具有较好的特异性和抗原结合活性,为了更好的阐明毕赤 酵母表达的重组人源性抗HBsAg Fab抗体的性质,用基质辅助激光解析飞行时间质谱(MALDI- TOF-MS)对重组Fab抗体的分子质量和肽质量图谱进行了分析。结果显示,毕赤酵母表达的重组 人源性抗HBsAg Fab抗体的分子质量为50678．49Da,与根据其一级结构计算的理论分子质量相 比多2763．84 Da,显示酵母表达的重组Fab抗体为糖蛋白。用胰蛋白酶酶解重组Fab抗体后进行 MALDI-TOF-MS分析显示,大部分的酶解肽段均能检测出来。结果表明毕赤酵母表达的重组Fab 抗体与预期的结构一致。The recombinant humanized anti-HBsAg Fab secreted from yeast cells has high antigen specificity and affinity activity, which could sufficiently neutralize the HBsAg. In order to clarify the character of the recombinant Fab, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry （MALDI-TOF-MS） was used to analyze the molecular mass and peptide mass spectrum. The results showed that the molecular mass of recombinant Fab was 50678.49 Da, which is 2763.84 Da higher than the calculated value. The recombinant Fab was treated by trypsin and analyzed by MALDI-TOF-MS. The most peptides could be found. The results indicated that the recombinant humanized anti-HBsAg Fab was constructed correctly.

关 键 词：重组Fab 分子质量 肽质量图谱 质谱 

分 类 号：Q78[生物学—分子生物学] TQ342.61[化学工程—化纤工业]