降解萘的假单胞菌ND24菌株的分离和萘污染土壤的生物修复  

Isolation of Naphthalene-Degrading Pseudomonas sp.ND24 and Bioremediation of Naphthalene-Contaminated Soils

作　　者：蔡宝立[1] 李永君[1] 梁靖[1] 赵化冰[1] 

机构地区：[1]南开大学生命科学学院,天津300071

出　　处：《食品与生物技术学报》2005年第6期6-9,共4页Journal of Food Science and Biotechnology

基　　金：国家自然科学基金项目(30270274)资助课题

摘　　要：用富集培养法从石油工业废水中分离到高效降解萘的ND24菌株.该菌株能以萘为惟一碳源生长,在48 h内将无机盐培养基中2 g/L的萘降解98.4%.该菌株还能降解水杨酸、对羟基苯甲酸、邻苯二甲酸和苯乙酸.16S rRNA基因测序和同源序列比对表明,ND24菌株属于假单胞菌(Pseudomonassp.).将ND24菌株接种到含有0.2 g/dL萘的灭菌土壤中,经过14 d室温培养以后,萘的去除率为98.2%.With the method of enrichment culture, a high efficient naphthalene-degrading strain, ND24, was isolated from petroleum wastewater. The strain was capable of utilizing naphthalene as a sole carbon source for growth and degrading naphthalene upto 98. 4 ~ of existed in minimal medium （2 g/L） within 48 hours. The strain also showed the capability of degrading salicylic acid, p-hydroxybenzoic acid, o-phthalic acid and phenylacetic acid. 16 S rRNA gene sequencing identified the strain ND24 as a Pseudornonas sp. After inoculating strain ND24 into the autoclaved soils containing 0.2% naphthalene and incubating it at room temperature for 14 days, 98.2% naphthalene was removed.

关 键 词：萘 生物降解 假单胞菌 土壤修复 

分 类 号：X172[环境科学与工程—环境科学]