H9N2亚型禽流感病毒陕西分离株的鉴定及HA、NP、NA全序列测定  

Isolation and Identification of Subtype H9N2 Avian Influenza Virus from Shaanxi Province and Sequence Analysis of It's HA、NP、NA Gene

作　　者：曹维伟[1] 陈杰[2] 张燕霞[2] 黄保续[2] 洪军[2] 陈德坤[1] 李晓成[2] 

机构地区：[1]西北农林科技大学动物科技学院,陕西杨凌712100 [2]农业部动物检疫所国家动物流行病学中心,山东青岛266032

出　　处：《中国动物检疫》2006年第1期25-27,43,共4页China Animal Health Inspection

摘　　要：从陕西地区疑似流感发病鸡分离到4株流感病毒,经国家流感中心鉴定均为A型流感病毒H9N2亚型。将其中1株A/Chicken/Shaanxi/3/2002(H9N2)的血凝素基因核蛋白基因和神经氨酸酶基因进行克隆和测序,与GenBank收录的其它流感H9N2亚型的相关基因进行比较,结果表明,A/Chicken/Shaanxi/3/2002(H9N2)的NA序列与A/Chicken/Shanghai/F/98(H9N2)的NA序列同源性较高,为98.9%,但其HA序列与香港人流感病毒A/HongKong/1073/99、A/HongKong/1074/99(H9N2)的HA序列同源性较低,为85.9%。另外,氨基酸进化树分析结果显示,A/Chicken/Shaanxi/3/2002(H9N2)与A/chicken/HongKong/CSW153/03(H9N2)的亲缘关系最近,两者形成独立的分支。Four strains of avian influenza virus were isolated from sick chickens in different farms of Shaanxi province from May 1998 to May 2003.They were all identified as influenza A virus of H9N2 subtype by National Influenza Research Center. The complete HA,NP,NA gene of one isolate A/Chicken/Shaanxi/3/ 2002（H9N2） was cloned,sequenced and compared with sequences available in GenBank. The results indicated that the isolate A/ChickerdShaanxi/3/2002（H9N2）possibly came from A/ChickerdShanghai/F/98.

关 键 词：H9N2亚型 禽流感病毒 HA NP NA 序列比较 

分 类 号：S852.659.5[农业科学—基础兽医学] S884.52[农业科学—兽医学]