儿茶素抑制兔晶状体上皮细胞增殖  

Inhibitory role of EGCG on proliferation of rabbit lens epithelial cells

作　　者：黄文勇[1] 曾骏文[1] 刘奕志[1] 吴明星[1] 

机构地区：[1]中山大学中山眼科中心,广东广州510060

出　　处：《中国病理生理杂志》2005年第12期2443-2446,共4页Chinese Journal of Pathophysiology

基　　金：广东省科技计划项目(NO.20030087)

摘　　要：目的:探讨在绿茶提取物表没食子儿茶素没食子酸酯(EGCG)抑制兔晶状体上皮细胞增殖过程中, MEK/ERK1/2(MITOGEN-ACTIVATED PROTEIN KINASE KINASE,MAPKK,MEK;EXTRACELLULAR SIGNAL-REGULATED KINASE,ERK)及C-JUN 氨基末端激酶(C-JUN NH2-TERMINAL PROTEIN KINASE,JNK)信号转导通路的作用。方法:实验按EGCG浓度分50、100和 200 ΜMOL/L3组。预先分别加入25、50 ΜMOL/L的ERK活性拮抗剂PD980059孵育1 H;用噻唑蓝比色法(MTT比色法) 测定晶状体上皮细胞增殖;用蛋白质免疫沉淀法(WESTERN BLOTTING)法检测ERK及JNK的磷酸化水平。结果:(1) PD980059能明显增强EGCG抑制晶状体上皮细胞增殖的作用,呈浓度依赖性。(2)晶状体上皮细胞内磷酸化的ERK 基础水平高。加入EGCG后其活性升高,随后逐渐回降,但始终高于基础水平;ERK的活化水平也随EGCG浓度的增加而增高。(3)细胞内JNK的54 KD亚型磷酸化的基础水平较低而46 KD亚型的较高;呈剂量与时间依赖性。结论: EGCG可能部分通过调节ERK和JNK的磷酸化水平而抑制晶状体上皮细胞的增殖。AIM： （ - ） - Epigallocatechin- 3 - gallate （EGCG） is an extract from green tea, it could strongly inhibit the growth of cultured rabbit lens epithelial cells. This study explored the role of MEK1/ERK1/2 and JNK pathways in the prolifera-, tion inhibition of rabbit lens epithelial ceils （LECs） induced by EGCG. METHODS： MTI＇ colormetric assay was used to study the LECs growth inhibition. Western blotting were applied to study the kinsae phnsphorylation level of ERK and JNK. RESULTS： （1） ERK activity blocker PD980059 could enhance EGCG - induced inhibition effect on proliferation of LECs compared with control groups in a dose - dependent manner. （2） Basic phnsphorylation level of ERK was fairy high in LECs; and was increased by EGCG in a dose - dependent manner. The ERK phosphorylation reached its peak immediately after EGCG was added and stayed high even at the end of the test. （3） The basic phosphorylation - level of 54 kD JNK was weak but 46 kD was high in LECs; EGCG increased phnsphorylated JNK level in a tinre and concentration - dependent manner in our test. CONCLUSION： It is suggested that the LEC proliferation inhibition induced by EGCG is, at least in part, through ERK and JNK pathways.

关 键 词：表没食子儿茶素没食子酸酯 晶状体上皮细胞 有丝分裂素激活蛋白激酶类 

分 类 号：R363[医药卫生—病理学]