反义hTR治疗联合Tamoxifen增强对人胶质瘤细胞生长的抑制作用  

Antisense hTR combined with Tamoxifen increase the inhibitory effect on growth of glioma cells

作　　者：尤永平[1] 浦佩玉[1] 黄强[1] 王春燕[1] 王广秀[1] 

机构地区：[1]天津医科大学总医院神经外科

出　　处：《中华神经外科杂志》2005年第12期751-753,共3页Chinese Journal of Neurosurgery

摘　　要：目的探讨Tamoxifen单独或联合端粒酶反义RNA(hTR)治疗对胶质瘤细胞生长的抑制作用;方法Tamoxifen单独或联合hTR反义cDNA作用于人胶质瘤细胞系TJ905细胞,用MTT 法、TRAP法、TUNEL法、免疫组化法等检测效果。结果 Tamoxifen、反义hTR治疗都能抑制 TJ905细胞生长,7d后细胞生存率分别为38．6％(TAM浓度为15mM)、52．8％,以二者联合应用抑制作用最强,细胞生存率为20．6％;凋亡指数明显增加,胶质瘤细胞内端粒酶活性、PKC和IGI-1 的表达被抑制。结论 hTR反义治疗联合Tamoxifen能通过抑制PKC和IGF-1表达增强对胶质瘤细胞生长的抑制作用。Objective To study the inhibitory effect of Tamoxifen and/or antisense hTR cDNA on glioma cells, Method The cultured human glioblastoma TJ905 cells were treated with Tamoxifen and/or antisense hTR cDNA,their inhibitory effect was evaluated by MTT assay. TRAP method. TUNEL method, immunohistochemistry, Results Tamoxifen or antisense hTR cDNA alone can inhibit the growth of glioma cells significantly,after 7 days, rate of survival of TJ905 cell was 38.6% （concentration of TAM was 15mM）, 52.8%,respectively. But the inhibitory effect was strongest during combined treatment with Tamoxifen and antisense hTR cDNA, The rate of survival of cell was 20.6%. Apoptotic index increased significant, Expressions of PKC and IGF-1 and telomerase activity were inhibited. Conclusions Antisense hTR combined with Tamoxifen can increase inhibitory effect on growth of glioma cells through inhibiting expression of PKC and IGF-1.

关 键 词：TAMOXIFEN 端粒酶 反义HTR 胶质瘤 

分 类 号：R739.4[医药卫生—肿瘤]