单剂量黄曲霉毒素B_1致大鼠肝癌作用的短期实验模型  

SHORT TERM EXPERIMENTAL ANIMAL MODEL OF HEPATOCEARCINOGENESIS INDUCED BY SINGLE DOSE of AFLATOXIN B1 IN RATS

作　　者：段小娴 覃柳亮 覃国忠 严瑞琪[1] 

机构地区：[1]广西肿瘤防治研究所病理研究室,中山医科大学肿瘤研究所

出　　处：《癌症》1996年第1期21-23,共3页Chinese Journal of Cancer

摘　　要：本文报告单剂量黄曲霉毒素Ｂ１（ＡＦＢ１）致大鼠肝癌作用短期实验模型的研究．６周龄、雄性Ｗｉｓｔａｒ大鼠，经腹腔一次性注射不同剂量（０、０．５０、０．７５、１．００和１．５０ｍｇ／ｋｇ体重）的ＡＦＢ１，作为启动剂。两周后，饲以含０．０１５％的２－乙酰氨基芴（２－ＡＡＦ）饲料４周。实验第三周末，施行肝大部分切除术（ＰＨ）。所有动物于实验第６周处死，取肝组织作Ｇａｍｍａ－谷氨酰转肽酶（ＧＧＴ）化学染色，然后对ＧＧＴ阳性肝细胞灶进行定量分析。结果显示单剂量ＡＦＢ１加２－ＡＡＦ及ＰＨ选择促进程序能诱发癌前肝细胞酶变灶，而ＡＦＢ１的启动作用有明显的剂量反应关系。A short term experiment for the induction of hepatocellullar precancerous lesions by singleof aflatoxin B1(AFB1) was studied.Five groups of 6-week-old inbred wistar Rats were given byI.P,withdifferent doses of AFB1(0,0.50,0.75,1.00,1.25 and 1.50mg/kg bw).Two groups of rats was served ascontrols given by DMSO only or without pretreatment.After two weeks,the animals were given by 0.015%2AAF-diet for four weeks.At the third week of experiment,partial hepatectomy was conducted.The ani-mals were sacrificed at sixth week.The liver tissues were processed for Gamma-glutamyl-transpeptidase(GGT)stain.The GGT postive hepotocytic hyperplastic foci were induced and revealed dose dependent ef-fect.

关 键 词：黄曲霉毒素B1 肝肿瘤 酶变灶 实验模型 

分 类 号：R735.702[医药卫生—肿瘤]