大黄鱼溃烂症的一种致病菌——奇异变形杆菌ZXS02菌株  

A pathogen isolated from skin-ulcer Pseudosciaena crocea——Proteus mirabilis ZXS02 strain

作　　者：张庆华[1] 熊清明[1] 肖琳琳[2] 杨灏[3] 杨先乐[1] 

机构地区：[1]上海水产大学生命科学与技术学院,上海200090 [2]中国水产科学研究院东海水产研究所,上海200090 [3]北京放射医学研究所,北京100850

出　　处：《水产学报》2005年第6期824-830,共7页Journal of Fisheries of China

基　　金：上海市教委青年基金项目(科04KC13)资助;上海市重点学科建设项目资助(Y1101);上海水产大学青年科研基金(科02-67);上海水产大学重点学科建设专项科研项目(科03-38)

摘　　要：从浙江象山网箱养殖的患体表溃烂症的大黄鱼(Pseudosciaena crocea)身上分离到7株细菌,人工感染试验表明分离菌株ZXS01、ZXS02、ZXS07为致病菌。常规生理生化测试表明,ZXS02菌株具有变形杆菌属(Proteus)的特征。为了进一步明确菌株ZXS02的分类学地位,测定其16S rRNA基因序列的1495个碱基,分析相关细菌相应序列的同源性,构建系统发生树,结果表明菌株ZXS02与奇异变形杆菌(Proteus mirabilis)的同源性可达99.0%。用Sensitire细菌鉴定系统对其进行验证,测试结果表明与奇异变形杆菌的相似性达99.96%。综合上述结果,可鉴定菌株ZXS02为奇异变形杆菌。同时该菌株的药敏试验、对盐度、温度的耐受性试验表明其对氯霉素等4种药物敏感,37℃、盐度20生长最适。Seven strains were isolated from Pseudosciaena crocea suffering from the skin-ulcer disease cultivated in Xiangshan Area of Zhejiang Province. Strain ZXS01 ,ZXS02 and ZXS07 were confirmed to be the pathogen of the disease by artificial infection test. The traditional physiological and biochemical methods showed that most of the phenotypes of strain ZXS02 were similar to Proteus. In order to investigate the phylogenetic position of this pathogen, 16S rRNA gene was sequenced, its molecular weight is 1495 bp. Molecular phylogenetic dendrogram was constructed based on the genetic distance analysis, which revealed that strain ZXS02 exhibited higher levels of 99.0% similarity to Proteus trdrabilis. Meanwhile, Sensitire （gram negative） identification system was applied in the determination of strain ZXS02, showing 99.96 % similarity with reference strain of P. mirabilis. Based on the results obtained from phenotypes and 16S rRNA gene analysis, strain ZXS02 was identified as P. mirabilis. Furthermore, its sensitivity to 23 antibiotics, temperature and salt were detected, the results showed that it was sensitive to chlommycetin and other 3 kinds of antibiotic, and the optimum temperature and salinity are 37℃ and 20 respectively.

关 键 词：大黄鱼 体表溃烂症 16S RRNA基因 电泳 PCR扩增 奇异变形杆菌 

分 类 号：S941.42[农业科学—水产养殖]