在MSMPR结晶器中谷氨酸的结晶动力学研究  

Study on the kinetics of crystallization of glutamic acid in the MSMPR crystallizer from the industrial fermentation broth

作　　者：丛进阳[1] 刘树中[1] 

机构地区：[1]清华大学化学工程系,北京100084

出　　处：《清华大学学报（自然科学版）》1996年第6期60-66,共7页Journal of Tsinghua University(Science and Technology)

摘　　要：用ＭＳＭＰＲ结晶器对谷氨酸从工业发酵液中连续等电点结晶的动力学进行了研究。 在假设△Ｌ定律适用于ＭＳＭＰＲ结晶过程的前提下，测量了晶体的粒数密度ｎ和晶体平均粒度 Ｌ之间的关系。在多组实验中１ｎ（ｎ·Ｌμｍ）和 Ｌ之间在Ｌ＞４０ μｍ范围内存在良好的直线关系， 说明谷氨酸结晶过程符合△Ｌ定律，在Ｌ＜４０ μｍ范围内，ｌｎ（ｎ·Ｌμｍ）值和Ｌ之间关系不符合 △Ｌ定律，对其原因在文中作了讨论。根据１ｎ（ｎ·Ｌμｍ）－Ｌ直线在坐标轴上的截距及其斜率所计 算出的晶核粒数密度ｎ°和晶体生长速率Ｇ之间的关系推导出结晶过程的成核速率Ｂ°。最后从 １ｎ（ｎ°·Ｌμｍ）与１ｎ（Ｇ·ｈμｍ－１）之间的直线关系再推导出谷氨酸从发酵液中连续等电点结晶在 １０℃情况下的成核速率Ｂ°－晶体生长速率Ｇ之间的动力学表达式：Ｂ°＝８．６５×１０7Ｇ０.２７。In this paper, MSMPR crystallizer was used to study the kinetics of continuous isoelectric point crystallization of glutamic acid from industrial fermentation broth. On the supposition that △L law is suitable for the MSMPR crystallization processes the relationship between the density of crystal particle-number n and the average particle-size L was measured. It showed that there was a good linear relation between ln n and L when L>40 μm, indicating that crystallization of glutamic acid conforms to the △L law. In the case of L <40 μm, the relationship between in n and L does not comply with the △L law. The reason for this phenomenon was discussed in this paper. The rate of nucleation B° in the crystallization was deduced from the linear relationship between the density of nucleus particle-number n° and the crystal growth rate G which were calculated from the intercept and the slope of in (n·Lμm)-L straight line. Finally, the kinetic formula of the rate of nucleation B° expressed by crystal growth rate G at 10℃ for the crystallization process was obtained from the linear relationship between in (n°. Lμm) and ln (G·hμm-1 ): B°＝8. 65× 107 G0． 27.

关 键 词：谷氨酸 MSMPR结晶器 结晶动力学 发酵液 

分 类 号：TQ922.1[轻工技术与工程—发酵工程]