一种新的真核细胞表达载体pEF－BOS的应用  

Transfection of COS cell using a new eukaryotic expression vector

作　　者：刘崟[1] 金伯泉[1] 

机构地区：[1]第四军医大学免疫学教研室

出　　处：《细胞与分子免疫学杂志》1996年第2期24-27,共4页Chinese Journal of Cellular and Molecular Immunology

摘　　要：采用一种新的真核细胞表达载体ｐＥＦ－ＢＯＳ在ＣＯＳ细胞内表达了ｇｐ１３０和ＧＡＭ两种分子，并利用免疫沉淀和Ｗｅｓｔｅｒｎ－ｂｌｏｔ观察了表达结果。发现应用ｐＥＦ－ＢＯＳ表达载体可在ＣＯＳ细胞内获得上述两种分子的较高表达，优于一般常用的ｐＳＶ表达载体。A new eukaryotic expression vector-pEF-BOS was used in the transfection of COScell, and the expression was detected by Western-blot and immunoprecipitation. It was foundthat PEF-BOS could give rise to a rather high expression, much better than the commonly usedpSV vector.

关 键 词：真核细胞 载体 表达 pEF-BOS 

分 类 号：R394.8[医药卫生—医学遗传学] Q782[医药卫生—基础医学]