神经肽注射液多肽成份的分离及鉴定  

The Isolation and Identification of Peptide Composition from Neuropeptide Injectio

作　　者：牛美娟[1] 李芳[1] 曲章义[1] 谷鸿喜[1] 韩庆宏[1] 

机构地区：[1]哈尔滨医科大学微生物教研室,哈尔滨医科大学生化教研室

出　　处：《生物技术》1996年第3期28-29,共2页Biotechnology

摘　　要：本实验采用ＳｅｐｈａｄｅｘＧ２５及ＡＣＡ凝胶柱层析技术，对神经肽注射液中多肽成份进行过柱分离。将过柱后收集的不同洗脱峰的溶液通过紫外扫描，茚三酮显色，微量双缩脲试验及薄层层析等技术进行鉴定。此外还用ＳＤＳ—ＰＡＧＥ检测该注射液纯度及分子量。结果表明神经肽注射液含有一种主要多肽成份及大量小分子肽类，其主要多肽成份的分子量为１４，０００Ｄ。The peptide components in neuropeptide injectio were separated with Sephadex G 25 and ACA gel filtration chromatography in this experiment. Then the liquid of different parts that were collected from gel column were identificated by UV scanning, ninhydrin, micro-- biuret reactions and thin--layer chromatography. Meanwhile, the purity and the molecular weight of the neuropeptide were determind by SDS -- Polyacrylamide gel electrophoresis, (SDS -- PAGE).The results showed that there were one main peptide component and some smaller molecular weight peptides in this injectic. The molecular weight of the main peptide component was about 14 , 400D.

关 键 词：神经肽注射液 多肽 分离 鉴定 生物制品 

分 类 号：R392－33[医药卫生—免疫学]