Polyethylenimine (PEI)g-comb-poly(ethylene glycol)-transferrin(I): Tumor-targeted Vector for Gene Delivery In-vitro  

作　　者：Gu Ping TANG Zhi Yu WANG 

机构地区：[1]College of Life-Science. ZheJiang University, Hangzhou 310028 [2]Institute of Materials Research ＆ Engineering, Singapore, 3 Research Link 117602 [3]The Second Hospital of Zhejiang University, Hangzhou 310006

出　　处：《Chinese Chemical Letters》2006年第1期113-116,共4页中国化学快报（英文版）

摘　　要：The work described the synthesis and evaluation of PEI-g-comb-PEG-transferrin as a potential system for gene therapy in vitro. The MW of PEG was 10KDa, and PEI was 2KDa. Its structure was identified by NMR, FT-IR and TGA spectroscopy. MTT assay found that at concentration up to 4000 n mol/L of the polymer, cell viability was over 85%. The bio-character of polymer/DNA complex was characterized by agarose gel electrophoresis, ethidium bromide exclusion and zeta-potential assay. The polymer could retardate DNA at N/P ratio 3.0-3.5 （mol/mol）. The particle size of the polymer/DNA complex was less than 300 nm. Transfection efficiency of the complex was studied in COS7 and NT2 cell lines.The work described the synthesis and evaluation of PEI-g-comb-PEG-transferrin as a potential system for gene therapy in vitro. The MW of PEG was 10KDa, and PEI was 2KDa. Its structure was identified by NMR, FT-IR and TGA spectroscopy. MTT assay found that at concentration up to 4000 n mol/L of the polymer, cell viability was over 85%. The bio-character of polymer/DNA complex was characterized by agarose gel electrophoresis, ethidium bromide exclusion and zeta-potential assay. The polymer could retardate DNA at N/P ratio 3.0-3.5 （mol/mol）. The particle size of the polymer/DNA complex was less than 300 nm. Transfection efficiency of the complex was studied in COS7 and NT2 cell lines.

关 键 词：PEI-g-Comb-PEG TRANSFERRIN gene therapy transfection efficiency. 

分 类 号：R730.5[医药卫生—肿瘤] R979.1[医药卫生—临床医学]