大豆磷脂脂质体对谷氨酸所致体外神经细胞损伤的保护作用  

Protective effects of soybean phospholipid liposomes on in vitro nerve cell injury induced by glutamate

作　　者：张新[1] 肖建英[1] 刘宇杰[1] 

机构地区：[1]锦州医学院生化教研室,辽宁省锦州市121001

出　　处：《中国临床康复》2006年第3期42-44,共3页Chinese Journal of Clinical Rehabilitation

摘　　要：目的:观察大豆磷脂脂质体对谷氨酸引起培养神经细胞兴奋毒性损伤的保护作用,并探讨其可能的作用机制。方法:①实验于2004-11/2005-06在锦州医学院生化实验室完成。选用清洁级新生0～1dSD大鼠12只,取大脑皮质神经细胞进行原代培养8～10d后,随机分为3组:正常对照组、损伤组和大豆磷脂脂质体保护组。损伤组加入谷氨酸(终浓度1×10-4mol/L)作用3h,造成神经细胞的损伤,大豆磷脂脂质体保护组在谷氨酸损伤处理的同时加入0.2,0.4,0.8,1.6g/L的大豆磷脂脂质体。②参照由南京建成生物工程研究所提供的试剂盒说明书测定培养液中乳酸脱氢酶活力和一氧化氮含量。培养神经细胞中丙二醛含量、超氧化物歧化酶及一氧化氮合酶活力测定参照由南京建成生物工程研究所提供的试剂盒说明书进行,蛋白质定量用考马斯亮兰试剂盒测定。③多样本均数检验采用方差分析的方法,方差分析差异有显著性后再进行样本的两两比较(Q检验)。结果:①培养上清液中乳酸脱氢酶活力、一氧化氮含量及培养神经细胞一氧化氮合酶活力:损伤组明显高于正常对照组(P<0.01),大豆磷脂脂质体各质量浓度保护组明显低于损伤组(P<0.01)。②培养神经细胞丙二醛含量:损伤组明显高于正常对照组(P<0.01),大豆磷脂脂质体各质量浓度保护组明显低于损伤组(P<0.01)。③培养神经细胞超氧化物歧化酶活力:损伤组明显低于正常对照组(P<0.01),大豆磷脂脂质体各质量浓度保护组明显高于损伤组(P<0.01)。大豆磷脂脂质体保护作用随着其质量浓度增加(0.2～0.8g/L)而增强,但当剂量达到一定浓度(1.6g/L),该保护作用不再随着浓度的升高而增强。结论:大豆磷脂脂质体对谷氨酸引起的培养神经细胞兴奋毒性损伤具有显著的保护作用,且存在大豆磷脂脂质体质量浓度依赖性;该种保护作用发生机制可能与大豆磷脂脂质体抗�AIM： To study the protective effects of soybeari phospholipid lipsomes （SPL） on glutamate （Glu）-induced neurotexicity in vitro and discuss its possible mechanisms. METHODS： （1） The experiment was completed in the Biochemistry Laboratory of Jinzhou Medical College from November 2004 to June 2005. Twelve Sprague-Dawley rats, 0-1 day old, were selected. The cortical neurons of neonatal rat were cultured in vitro for 8 or 10 days, and rats were divided into three groups： normal control group, injury group and SPL group. Glu （1×10^-4mol/L^-1） was used to act on neurocytes in injury group for 3 hours, while different concentrations of SPL （0.2, 0.4, 0.8, 1.6 g/L） were added at the same time in SPL group. （2）According to the reagent box manual of Nanjing Jiancheng Bioengineering Research Institute, activity of lactate dehydrogenase （LDH） and nitric oxide （NO） content were assayed in the supematant of the cultfire medium and also the activity of nitric oxide synthase （NOS）, superoxide dismutase （SOD）, maleic dialdehyde （MDA） content in the neurocytes. Protein ration was assayed with Kaomaslianglan reagent box. （2） Variance analysis was used to test mean of many samples, and the differences of two samples were compared after variance analysis so as to show distinct differences （Q test）. RESULTS： （1） Activity of LDH, content of NO in the supematant of the culture medium and activity of NOS in the neurocytes： Those in injury group were obviously higher than those in normal control group （P〈0.01）, but those in SPL group were lower than those in injury group （P〈0.01）. （2） Content of MDA in the neurocytes： Those in injury group were obviously higher than those in normal control group （P〈0.01）, but those in SPL group were lower than those in injury group （P〈0.01）. （3） Activity of SOD in the neurocytes： Those in injury group were obviously lower than those in normal control group （P〈0.01）, but those in SPL group were

关 键 词：脑缺血 磷脂类 脂质体 谷氨酸 细胞培养 大豆磷脂脂质体 体外神经细胞损伤 保护作用 

分 类 号：R743[医药卫生—神经病学与精神病学]