PTEN基因表达与胃癌细胞侵袭力的相关性研究  

Relationship between the expression of tumor suppressor PTEN gene and the invasiveness of gastric cancer

作　　者：邓全军[1] 于皆平[1] 周燕红[1] 夏虹[1] 于红刚[1] 

机构地区：[1]武汉大学人民医院,湖北武汉430060

出　　处：《山东医药》2006年第1期15-17,共3页Shandong Medical Journal

基　　金：国家自然科学基金资助项目(30300154)

摘　　要：目的探讨PTEN基因mRNA表达与胃癌细胞体外侵袭力的关系。方法采用Boyden小室法测定四种胃癌细胞(SGC-7901、BGC-823、M GC-803、HGC-27)的体外侵袭力、运动力,RT-PCR法检测四种胃癌细胞的PTEN基因mRNA表达水平。结果胃癌细胞体外侵袭力为HGC-27(48±2)、M GC-803(28±2)、BGC-823(30±3)、SGC-7901(13±2);PTEN基因mRNA表达水平为SGC-7901(0.336±0.079)、BGC-823(0.232±0.063)、M GC-803(0.228±0.056)、HGC-27(0.113±0.047);两两比较,P<0.01,>0.05,<0.05。相关分析显示,胃癌细胞体外侵袭力随PTEN基因mRNA表达增高而降低,PTEN基因mRNA表达及胃癌细胞体外侵袭力与细胞分化程度相关。结论PTEN基因参与胃癌的发展过程,并与胃癌的部分生物学行为有关,PTEN基因mRNA表达水平对评价胃癌细胞体外运动侵袭力具有重要价值。Objective： To observe the relationship between the expression of tumor suppressor PTEN gene and the invasiveness of gastric cancer cell lines in vitro. Methods： The expression of PTEN mRNA and the invasiveness and motility of 4 gastric cancer cell lines （SGC-7901, BGC-823, MGC-803, HGC-27） were measured by semi-quantitative RT-PCR and Boyden chamber, respectively. ResultS： The invasiveness of 4 cancer ceil lines in magnitude order in vitro were HGC-27 （48±2）, MGC-803 （28± 2）, BGC-823 （30±3）, SGC -7901 （13±2）; The PTEN mRNA expression of these cancer cell lines in magnitude order were SGC-7901 （ 0. 336 ± 0. 079）, BGC-823 （0. 232 ± 0, 063）, MGC-803 P 〉 0, 05, P 〈= 0. 05 can be found respectively when （0. 228±0. 056）, HGC-27 （0. 113±0. 047）; P〈0. 01, compared among them. The data suggested that the invasiveness of gastric cancer cell degraded gradually following the increase of the expression level of PTEN mRNA. In addition, the invasiveness of gastric cancer cell lines and PTEN mRNA expression were related with the differentiation level of cancer cell. Conclusion： PTEN might play some roles in the development of gastric cancer, and was related with partial biological behavior. Determination of PTEN expression level was of great value in evaluating the motility and invasiveness of gastric cancers.

关 键 词：PTEN基因 胃癌细胞 肿瘤侵袭力 运动力 

分 类 号：R735.2[医药卫生—肿瘤]