氮磷营养盐对微氏海链藻细胞生化组成的影响  

Effects of nitrate and phosphate on the biochemical composi-tion of Thalassiosira weissflogii

作　　者：李顺兴[1] 郑凤英[2] 洪华生[1] 黄邦钦[1] 王大志[1] 林慧琴[2] 林玲[2] 

机构地区：[1]厦门大学近海海洋环境科学国家重点实验室,福建厦门361005 [2]漳州师范学院化学系,福建漳州363000

出　　处：《海洋科学》2006年第1期49-53,共5页Marine Sciences

基　　金：国家自然科学基金资助项目(40131160735;40506020);福建省科技三项费用资助项目(K02093);福建省青年科技人才创新项目(2003J035);福建省教育厅A类项目(JA00233)

摘　　要：探讨了高营养盐条件下氮浓度及氮磷摩尔比[n(N):n(P)]异动对微氏海链藻(Thalassiosiraweissflogii)的细胞碱性活性基团含量、细胞干质量、细胞形态及细胞体中碳水化合物、蛋白质、叶绿素含量,即对细胞生命力和表面吸附力的影响。结果表明,n(N):n(P)=16时,叶绿素、碳水化合物和蛋白质含量最高,生长状况及生命力最佳,有利于金属吸收,n(N):n(P)偏离16,各组份含量均减少。碱性活性基团和藻细胞大小,当n(N):n(P)=8时最大,表面吸附金属能力最强;n(N):n(P)=64时最小,不利于金属的吸附。氮磷摩尔比对微氏海链藻细胞体主动吸收和生物吸附金属的影响不同。Thalassiosira weissflogii was cultured in high levels of nutrients, and the influence of different levels of two macro-nutrients, nitrate and phosphate on biochemical composition, including the contents of carbohydrate, protein, chlorophyll, basic function group, cell dry weight and cell shape was investigated to assess the bioactivity, surface adsorption capability, i.e., the metal uptake/adsorption ability by T. weissflogii. When the ratio of nitrate to phosphate [n（N）：n（P）] is 16, the contents of chlorophyll, carbohydrate, and protein were the highest, its growth level and bioactivity were the best, and its ability to absorb the trace element were also outstanding. But the quantity of basic function group in cellular wall and cell size was the highest when n（N）：n（P） is 8, the lowest when n（N）：n（P） is 64. So, the ability for bio-absorption trace element is good when n（N）：n（P） is 8, and poor when n（N）：n（P） is 64. So, the influences of n（N）：n（P） on the metal uptake and adsorption were quite different.

关 键 词：营养盐 生化组成 植物营养学 海洋浮游植物 微氏海链藻(Thalassiosira weissfiogii) 

分 类 号：Q946[生物学—植物学]