大蒜素对人胃癌细胞SGC-7901及BGC-823G_2/M期的阻滞调节机制  

Inhibition of Allicin on G_2/M phase of human gastric cell line SGC-7901 and BGC-823

作　　者：柳星[1] 马锐[1] 陈威[1] 白雪蕾[1] 袁媛[1] 

机构地区：[1]中国医科大学附属第一医院肿瘤研究所第三研究室,辽宁省沈阳市110001

出　　处：《世界华人消化杂志》2005年第20期2409-2412,共4页World Chinese Journal of Digestology

基　　金：十五国家科技攻关项目;No.2004BA703B04-02~~

摘　　要：目的:研究大蒜素对人胃癌细胞SGC-7901及BGC-823G2/M期阻滞作用及其调节机制.方法:应用MTT法测定大蒜素对人胃癌细胞株SGC-7901和BGC-823细胞增殖抑制率及72h的IC50.通过流式细胞仪检测IC50浓度的大蒜素对SGC-7901和BGC-823细胞周期的影响.应用免疫组化染色检测大蒜素作用前后SGC-7901和BGC-823细胞CDC2和CyclinB蛋白表达.结果:不同浓度的大蒜素可以抑制人胃癌细胞株SGC-7901和BGC-823的增殖,且随着大蒜素浓度的增大,抑制率逐渐增高.大蒜素抑制SGC-7901细胞增殖50%的药物浓度(IC50):72h为23mg/L;大蒜素抑制BGC-823细胞增殖50%的药物浓度(IC50):72h为35mg/L.大蒜素作用于两种细胞,其细胞周期均发生了明显的变化,主要表现为G0/G1期细胞减少,G2/M期细胞增多(SGC-7901细胞24,48hvs对照:26.47±2.54%,28.88±2.75%vs24.30±2.74%,P<0.01;BGC-823细胞24,48hvs对照:22.78±1.45%,24.87±1.61%vs20.32±1.34%,P<0.01),S期细胞无明显变化.未经大蒜素处理的两种细胞,CDC2和CyclinB蛋白表达均为阳性,大蒜素处理后,CDC2和CyclinB蛋白表达下降.SGC-7901细胞经23mg/L大蒜素处理后,CDC2蛋白相对阳性表达率为87.2%;CyclinB蛋白相对阳性表达率为59.3%.BGC-823细胞CDC2蛋白在35mg/L大蒜素作用后,相对阳性表达率为84.4%;CyclinB蛋白相对阳性表达率为62.8%,与对照组相比均有显著性差异(P<0.01).结论:大蒜素使人胃癌细胞株SGC-7901和BGC-823停滞于G2/M期,其机制是通过CDC2和CyclinB蛋白表达下降实现的.AIM： To explore the inhibitory effect of Allicin on the G2/M phase of human gastric cell line SGC-7901 and BGC-823 and its mechanism. METHODS： The proliferation inhibition rate and IC5o of Allicin （72 h） to SGC-7901 and BGC-823 cells were examined by MTT assay. The cycles of the cells were measured by flow cytometry when IC50 concentration of Allicin was adopted. The expression of CDC2 and CyclinB protein were detected by immunohistochemistry. RESULTS： Allicin inhibited the proliferation of SGC-7901 （IC50 = 23 mg/L） and BGC-823 （I50 = 35 mg/L） cells and the inhibition rate was elevated with the increase of Allicin concentration. After treatment with ICso concentration of Allicin, the percentage of Go/G1 phase cells was decreased, but that of G2/M phase ones was significantly increased for both SGC-7901 （,24, 48 h vs control： 26.47±2.54%, 28.88±2.75% vs 24.30±2.74%, P 〈0.01） and BGC-823 （24, 48 h vs control： 22.78±1.45%, 24.87±1.61% vs 20.32±1.34%, P 〈0.01）. CDC2 and CyclinB proteins were positively expressed in SGC-7901 and BGC-823 cells without Allicin treatment. After treatment, the comparative rates of CDC2 and CyclinB expression were 87.2% and 59.3% in SGC-7901 cells, respectively, and 84.4% and 62.8% in BGC-823 cells, respectively （all P 〈0.01）. CONCLUSION： Allicin causes arrest of SGC-7901 and BGC-823 cells in G2/M phase, and the mechanism is related to the down-regulation of CDC2 and CyclinB expression.

关 键 词：大蒜素 SGC-7901 BGC-823 G2/M期阻滞 CDC2 CyclinB 人胃癌细胞SGC-7901 BGC-823细胞 阻滞作用 M期 

分 类 号：R735.2[医药卫生—肿瘤]