Synergistic action of famotidine and chlorpheniramine on acetic acid-induced chronic gastric ulcer in rats  

作　　者：Zhen Qin Chao Chen 

机构地区：[1]Laboratory of natural drugs of Medical College, China Three Gorges University, Yichang 443002, Hubei Province, China

出　　处：《World Journal of Gastroenterology》2005年第45期7203-7207,共5页世界胃肠病学杂志（英文版）

基　　金：Supported by Scientific and Technological Offices of Yichang Municipality, No A03210

摘　　要：AIM： To assess the synergistic action of famotidine （FMD） and chlorpheniramine （CPA） on acetic acid-induced chronic gastric ulcer in rats. METHODS： Chronic gastric lesions were induced in male Sprague-Dawley （SD） rats by serosal application of the acetic acid. Forty SD rats were randomly divided into blank group （n = 8）, control group （n = 8）, FMD group （n = 8）, CPA group （n = 8）, and FMD＋CPA group （n = 8）. Each group was given intraperitoneally （i.p.） 0.5 mL/100 g distilled water, 9 g/L NaCl saline, 4 mg/kg FMD, 10 mg/kg CPA, 4 mg/kg FMD＋10 mg/kg CPA, respectively, daily for 10 d, On d 10, ulcer area was determined by planimetry, The level of myeloperoxidase （MPO） in the liver homogenation was determined by biochemical methods and the plasma levels of 6-ketoprostaglandin F1 alpha （6-keto-PGFla）and IL-8 were determined by radioimmunoassay. RESULTS： The synergistic effects of FMD＋CPA group on the lesion, IL-8, 6-keto-PGFla and MPO were confirmed. The effect of FMD＋CPA group was significantly different as compared to the control and FMD groups. The lesion （mm2） was reduced from 40.18±2.6 in control group to 6.83±2.97 in PMD＋CPA group, P〈0.01, and from 32.9 ±3.27 in FMD group to 6.83±2.97 in PMD＋CPA group, P〈0.01. The plasma levels of IL-8 decreased from 0.69± 0.11 ng/L in control group to 0.4±0.04 ng/L in PMD＋CPA group, P〈0.01, and from 0.51±0.08 ng/L in FMD group to 0.4±0.04 ng/L in PMD＋CPA group, P〈0.05. The level of 6-keto-PGF1, increased from 7.55±1.65 ng/L in control group to 16.62±0.97 ng/L in PMD＋CPA group,P〈0.01, and from 13.15±1.48 ng/L in FMD group to 16.62±0.97 ng/L in PMD＋CPA group,P〈0.05. The levels of MPO in the liver homogenate decreased from 9.12±2.05 u/Lin control group to 4.33±0.95 u/L in PMD＋CPA group, P〈0.01, and from 8.3±1.29 u/L in FMD group to 4.33±0.95 u/L, P〈0.01. CONCLUSION： The synergistic action of FMD and CPA on acetic acid-induced chronic gastric ulcer in rats decreases the incAIM: To assess the synergistic action of famotidine (FMD)and chlorpheniramine (CPA) on acetic acid-induced chronic gastric ulcer in rats.METHODS: Chronic gastric lesions were induced in male Sprague-Dawley (SD) rats by serosal application of the acetic acid. Forty SD rats were randomly divided into blank group (n = 8), control group (n = 8), FMD group (n= 8), CPA group (n = 8), and FMD+CPA group (n = 8).Each group was given intraperitoneally (i.p.) 0.5 mL/100g distilled water, 9 g/L NaCl saline, 4 mg/kg FMD, 10mg/kg CPA, 4 mg/kg FMD+10 mg/kg CPA, respectively,daily for 10 d. On d 10, ulcer area was determined by planimetry. The level of myeloperoxidase (MPO) in the liver homogenation was determined by biochemical methods and the plasma levels of 6-ketoprostaglandin F1 alpha (6-keto-PGF1a)and IL-8 were determined by radioimmunoassay.RESULTS: The synergistic effects of FMD+CPA group on the lesion, IL-8, 6-keto-PGF1a and MPO were confirmed.The effect of FMlD+CPA group was significantly different as compared to the control and FMD groups. The lesion (mm2) was reduced from 40.18±2.6 in control group to 6.83±2.97 in PMD+CPA group, P＜0.01, and from 32.9±3.27 in FMD group to 6.83±2.97 in pMlD+CPA group,P＜0.01. The plasma levels of IL-8 decreased from 0.69±0.11 ng/L in control group to 0.4±0.04 ng/L in PMD+CPA group, P＜0.01, and from 0.51±0.08 ng/L in FMD group to 0.4±0.04 ng/L in PMD+CPA group, P＜0.05. The level of 6-keto-PGF1a increased from 7.55±1.65 ng/L in control group to 16.62±0.97 ng/L in PMD+CPA group, P＜0.01,and from 13.15±1.48 ng/L in FMD group to 16.62±0.97ng/L in PMD+CPA group, P＜0.05. The levels of MPO in the liver homogenate decreased from 9.12±2.05 u/Lin control group to 4.33±0.95 u/L in PMD+CPA group,P＜0.01, and from 8.3±1.29 u/L in FMD group to 4.33±0.95 u/L, P＜0.01.CONCLUSION: The synergistic action of FMD and CPA on acetic acid-induced chronic gastric ulcer in rats decreases the incidence of ulcer and also enhances the healing of ulcer.

关 键 词：Gastric ulcer Acetic acid FAMOTIDINE CHLORPHENIRAMINE INTERLEUKIN-8 6-Ketoprostaglandin F1 alpha MYELOPEROXIDASE 

分 类 号：R573[医药卫生—消化系统]