硫酸盐还原菌产亚硫酸盐还原酶的发酵工艺研究  

Study on the Fermentation Technology of Sulphate Reducing Bacteria Producing Sulfite Reductase

作　　者：刘晓华[1] 万海清[1] 杨睿[1] 贺庆[1] 

机构地区：[1]四川大学化学工程学院,成都610065

出　　处：《四川大学学报（自然科学版）》2006年第1期212-216,共5页Journal of Sichuan University(Natural Science Edition)

基　　金：国家自然科学基金(20076030)

摘　　要：对硫酸盐还原菌产亚硫酸盐还原酶的发酵工艺条件进行了研究,主要内容包括碳源、氮源、温度、起始pH、装液量及发酵周期等.通过单因素实验研究表明,以可溶性淀粉为碳源,以尿素作为氮源,选C∶N比为1∶3,在初始pH 7.0的条件下,接种量为5%,装液量为1∶3,35℃摇床培养42h,酶活力可达到16.2U/mL.The fermentation conditions of sulphate reducing bacteria producing sulfite reductase were reported, including carbon sources, nitrogen sources, temperature, pH, the loading amount and so on. Through the single factor experiments, it was indicated the culture for sulfite reductase production consisted of soluble starch, urea, the C ： N rate of 1 ： 3. After fermentation at pH 7.0, 5 percent of inoculating amount, with loading amount of 1 ： 3 and fermented at 35℃ for 42 hours. The enzyme activity was about 16.2U/mL.

关 键 词：亚硫酸盐还原酶 条件 酶活力 

分 类 号：Q93[生物学—微生物学]